Function of Ca2+ influx in phospholipase D activation induced by prostaglandin F in osteoblast-like cells: Involvement of tyrosine kinase

O. Kozawa, A. Suzuki, Y. Watanabe, J. Shinoda, Y. Oiso

研究成果: Article査読

7 被引用数 (Scopus)

抄録

We previously reported that prostaglandin F (PGF) induces Ca2+ influx from the extracellular space via protein tyrosine kinase in osteoblast-like MC3T3-E1 cells and that PGF stimulates phosphatidylcholine-hydrolyzing phospholipase D in these cells (6, 12). In this study, we examined the relationship between the tyrosine kinase-regulated Ca2+ influx by PGF and the activation of phospholipase D in MC3T3-E1 cells. The depletion of extracellular Ca2+ by [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) markedly reduced the PGF-induced formation of choline. Genistein, an inhibitor of protein tyrosine kinases, which by itself had little effect on choline formation, significantly suppressed the formation of choline induced by PGF in a dose-dependent manner. Tyrphostin, an inhibitor of protein tyrosine kinases chemically distinct from genistein, also suppressed the PGF-induced formation of choline. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, significantly enhanced the PGF-induced formation of choline. These results strongly suggest that the phospholipase D activation by PGF is dependent on extracellular Ca2+ in osteoblast-like cells and that protein tyrosine kinase is involved in the activation of phospholipase D.

本文言語English
ページ(範囲)319-323
ページ数5
ジャーナルProstaglandins, Leukotrienes and Essential Fatty Acids
52
5
DOI
出版ステータスPublished - 05-1995
外部発表はい

All Science Journal Classification (ASJC) codes

  • 臨床生化学
  • 細胞生物学

フィンガープリント

「Function of Ca<sup>2+</sup> influx in phospholipase D activation induced by prostaglandin F<sub>2α</sub> in osteoblast-like cells: Involvement of tyrosine kinase」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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