Functional alterations of human platelets following 111In labeling with different ligands and incubation media

M. Mieno, Y. Isaka, K. Kimura, M. Matsumoto, H. Etani, A. Uehara, K. Hashikawa, R. Hata, H. Moriwaki, K. Ashida, M. Imaizumi, T. Kamada, T. Kozuka

研究成果: Article

抜粋

We studied the effects of various 111In-water soluble chelates and incubation media on labeling efficiency of platelets and in vitro platelet aggregability. High labeling efficiency of platelets in ACD-saline was achieved with 111In-oxine sulfate, 111In-tropolone and 111In-MPO (2-mercaptopyridine-N-oxide). In the condition with 4.8 x 106/mm3 platelets in ACD-plasma, 111In-oxine-sulfate had low labeling efficiency and inconsistent labeling, while 111In-tropolone and 111In-MPO had high labeling efficiency. In vitro platelet aggregability (ADP 2 μM) was reduced when platelets were labeled in the absence of plasma. However, there was no significant difference in platelet aggregability among 111In-platelets labeled by three different chelates. In conclusion, to maintain aggregation activity of the platelets with relatively high labeling efficiency, the best result was obtained by using MPO or tropolone chelate in plasma at 4.8 x 106/μl platelet concentration.

元の言語English
ページ(範囲)443-449
ページ数7
ジャーナルKakuigaku
27
発行部数5
出版物ステータスPublished - 01-01-1990
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

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  • これを引用

    Mieno, M., Isaka, Y., Kimura, K., Matsumoto, M., Etani, H., Uehara, A., Hashikawa, K., Hata, R., Moriwaki, H., Ashida, K., Imaizumi, M., Kamada, T., & Kozuka, T. (1990). Functional alterations of human platelets following 111In labeling with different ligands and incubation media. Kakuigaku, 27(5), 443-449.