Functional alterations of human platelets following 111In labeling with different ligands and incubation media

M. Mieno, Y. Isaka, K. Kimura, M. Matsumoto, H. Etani, A. Uehara, K. Hashikawa, R. Hata, H. Moriwaki, K. Ashida, M. Imaizumi, T. Kamada, T. Kozuka

研究成果: Article査読

抄録

We studied the effects of various 111In-water soluble chelates and incubation media on labeling efficiency of platelets and in vitro platelet aggregability. High labeling efficiency of platelets in ACD-saline was achieved with 111In-oxine sulfate, 111In-tropolone and 111In-MPO (2-mercaptopyridine-N-oxide). In the condition with 4.8 x 106/mm3 platelets in ACD-plasma, 111In-oxine-sulfate had low labeling efficiency and inconsistent labeling, while 111In-tropolone and 111In-MPO had high labeling efficiency. In vitro platelet aggregability (ADP 2 μM) was reduced when platelets were labeled in the absence of plasma. However, there was no significant difference in platelet aggregability among 111In-platelets labeled by three different chelates. In conclusion, to maintain aggregation activity of the platelets with relatively high labeling efficiency, the best result was obtained by using MPO or tropolone chelate in plasma at 4.8 x 106/μl platelet concentration.

本文言語English
ページ(範囲)443-449
ページ数7
ジャーナルKakuigaku
27
5
出版ステータスPublished - 01-01-1990
外部発表はい

All Science Journal Classification (ASJC) codes

  • 放射線学、核医学およびイメージング

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