Functional regulation of GATA-2 by acetylation

Fumihiko Hayakawa, Masayuki Towatari, Yukiyasu Ozawa, Akihiro Tomita, Martin L. Privalsky, Hidehiko Saito

研究成果: Article査読

85 被引用数 (Scopus)

抄録

The transcription factor GATA-2 is expressed in hematopoietic stem and progenitor cells and is functionally implicated in their survival and proliferation. In the present study, we show that GATA-2 exists as an acetylated protein in immature precursor cells, KG1. GATA-2 was acetylated in vitro by p300 and GCN5. We have identified multiple acetylation sites by p300 on GATA-2, which include sites outside the zinc finger domain. We confirmed that GATA-2 acetylation occurred in transiently transfected 293T cells at sites similar to those induced by p300 in vitro. We have successfully shown that acetylation of GATA-2 in vitro increased its DNA-binding activity. In addition, GATA-2 displayed a transcriptional synergism with p300 that was impaired by mutation of each acetylation site. More importantly, each mutation in the acetylation sites of GATA-2 abolished its growth inhibitory effect on an interleukin-3-dependent progenitor, 32D. We conclude that acetylation provides multiple control points for the regulation of GATA-2 function.

本文言語English
ページ(範囲)529-540
ページ数12
ジャーナルJournal of Leukocyte Biology
75
3
DOI
出版ステータスPublished - 03-2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学
  • 細胞生物学

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