Gene transfer of dominant negative Rho kinase suppresses neointimal formation after balloon injury in pigs

Yasuhiro Eto, Hiroaki Shimokawa, Junko Hiroki, Kunio Morishige, Tadashi Kandabashi, Yasuharu Matsumoto, Mutsuki Amano, Masahiko Hoshijima, Kozo Kaibuchi, Akira Takeshita

研究成果: Article査読

89 被引用数 (Scopus)

抄録

Restenosis after angioplasty still remains a major problem for which neointimal formation appears to play an important role. Recent studies in vitro suggested that Rho kinase, a target protein of Rho, is important in various cellular functions. We thus examined whether Rho kinase is involved in the restenotic changes after balloon injury. In vivo gene transfer was performed immediately after balloon injury in both sides of the porcine femoral arteries with adenoviral vector encoding either a dominant negative form of Rho kinase (AdDNRhoK) or β-galactosidase (AdLacZ) as a control. One week after the transfer, immunohistochemistry confirmed the successful gene expression in the vessel wall, whereas 2 wk after the transfer, Western blotting showed the functional upregulation of Rho kinase at the AdLacZ site and its suppression at the AdDNRhoK site. Angiography showed the development of a stenotic lesion at the AdLacZ site where histological neointimal formation was noted, whereas those changes were significantly suppressed at the AdDNRhoK site. These results indicate that Rho kinase is involved in the pathogenesis of neointimal formation after balloon injury in vivo.

本文言語English
ページ(範囲)H1744-H1750
ジャーナルAmerican Journal of Physiology - Heart and Circulatory Physiology
278
6 47-6
DOI
出版ステータスPublished - 06-2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生理学
  • 循環器および心血管医学
  • 生理学(医学)

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