General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer

Qiang Sun, Akila Mayeda, Robert K. Hampson, Adrian R. Krainer, Fritz M. Rottman

研究成果: Article

234 引用 (Scopus)

抄録

The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA. More importantly, SF2/ASF stimulates in vitro splicing of bGH intron D through specific interaction with the ESE sequences. However, another general splicing factor, SC35, does not bind the ESE sequences and has no effect on bGH intron D splicing. Thus, one possible function of SF2/ASF in alternative and, perhaps, constitutive pre-mRNA splicing is to recognize ESE sequences. The stimulation of bGH intron D splicing by SF2/ASF is counteracted by the addition of hnRNP A1. The relative levels of SF2/ASF and hnRNP A1 influence the efficiency of bGH intron D splicing in vitro and may be the underlying mechanism of this alternative pre-mRNA processing event in vivo.

元の言語English
ページ(範囲)2598-2608
ページ数11
ジャーナルGenes and Development
7
発行部数12 PART B
出版物ステータスPublished - 01-12-1993
外部発表Yes

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Alternative Splicing
Introns
RNA Precursors
Exons
RNA Splicing Factors
bovine growth hormone
In Vitro Techniques
hnRNP A1

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Genetics

これを引用

Sun, Q., Mayeda, A., Hampson, R. K., Krainer, A. R., & Rottman, F. M. (1993). General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. Genes and Development, 7(12 PART B), 2598-2608.
Sun, Qiang ; Mayeda, Akila ; Hampson, Robert K. ; Krainer, Adrian R. ; Rottman, Fritz M. / General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. :: Genes and Development. 1993 ; 巻 7, 番号 12 PART B. pp. 2598-2608.
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abstract = "The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA. More importantly, SF2/ASF stimulates in vitro splicing of bGH intron D through specific interaction with the ESE sequences. However, another general splicing factor, SC35, does not bind the ESE sequences and has no effect on bGH intron D splicing. Thus, one possible function of SF2/ASF in alternative and, perhaps, constitutive pre-mRNA splicing is to recognize ESE sequences. The stimulation of bGH intron D splicing by SF2/ASF is counteracted by the addition of hnRNP A1. The relative levels of SF2/ASF and hnRNP A1 influence the efficiency of bGH intron D splicing in vitro and may be the underlying mechanism of this alternative pre-mRNA processing event in vivo.",
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Sun, Q, Mayeda, A, Hampson, RK, Krainer, AR & Rottman, FM 1993, 'General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer', Genes and Development, 巻. 7, 番号 12 PART B, pp. 2598-2608.

General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. / Sun, Qiang; Mayeda, Akila; Hampson, Robert K.; Krainer, Adrian R.; Rottman, Fritz M.

:: Genes and Development, 巻 7, 番号 12 PART B, 01.12.1993, p. 2598-2608.

研究成果: Article

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T1 - General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer

AU - Sun, Qiang

AU - Mayeda, Akila

AU - Hampson, Robert K.

AU - Krainer, Adrian R.

AU - Rottman, Fritz M.

PY - 1993/12/1

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N2 - The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA. More importantly, SF2/ASF stimulates in vitro splicing of bGH intron D through specific interaction with the ESE sequences. However, another general splicing factor, SC35, does not bind the ESE sequences and has no effect on bGH intron D splicing. Thus, one possible function of SF2/ASF in alternative and, perhaps, constitutive pre-mRNA splicing is to recognize ESE sequences. The stimulation of bGH intron D splicing by SF2/ASF is counteracted by the addition of hnRNP A1. The relative levels of SF2/ASF and hnRNP A1 influence the efficiency of bGH intron D splicing in vitro and may be the underlying mechanism of this alternative pre-mRNA processing event in vivo.

AB - The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA. More importantly, SF2/ASF stimulates in vitro splicing of bGH intron D through specific interaction with the ESE sequences. However, another general splicing factor, SC35, does not bind the ESE sequences and has no effect on bGH intron D splicing. Thus, one possible function of SF2/ASF in alternative and, perhaps, constitutive pre-mRNA splicing is to recognize ESE sequences. The stimulation of bGH intron D splicing by SF2/ASF is counteracted by the addition of hnRNP A1. The relative levels of SF2/ASF and hnRNP A1 influence the efficiency of bGH intron D splicing in vitro and may be the underlying mechanism of this alternative pre-mRNA processing event in vivo.

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