Generation and characterization of Autographa californica nucleopolyhedrovirus mutants defective in pcna gene homologue

Satoko Iwahori, Motoko Ikeda, Michihiro Kobayashi

研究成果: ジャーナルへの寄稿学術論文査読

8 被引用数 (Scopus)

抄録

Two Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) mutants defective in conserved pcna 1 and pcna 2 sequences and in substantial portion of the C-terminal region of pcna open reading frame were generated. Biological characterization revealed that these pcna-defective mutants were both viable in the cell culture and the kinetics of viral DNA replication, viral structural protein synthesis, budded virion yield and polyhedrin production in the pcna-defective AcMNPV-infected cells were comparable to those in the cells infected with wild-type (wt) AcMNPV. Localization experiments showed that the amount of cellular PCNA in the nuclear fraction was significantly higher in the cells infected with pcna-defective AcMNPVs than in the cells infected with wt AcMNPV from 8 to 16 h postinfection. These results indicate that AcMNPV PCNA is not essential not only for viral DNA replication but also for the yield of progeny virions and suggest that cellular PCNA functions in the viral DNA replication in the cells infected with pcna-defective AcMNPV mutants. On the basis of our data from cellular PCNA localization experiments and the fact that pcna-missing NPVs are able to multiply to high titers in the cell culture, it is also suggested that virus-encoded PCNA is crucial for viral DNA replication only in the cells that provide an environment unfavorable for DNA replication.

本文言語英語
ページ(範囲)129-139
ページ数11
ジャーナルJournal of Insect Biotechnology and Sericology
71
3
出版ステータス出版済み - 10-2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー
  • ビジネス、管理および会計一般
  • 農業および生物科学一般
  • 昆虫科学
  • 産業および生産工学

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