Generation of infectious recombinant human rotaviruses from just 11 cloned cDNAs encoding the rotavirus genome

Satoshi Komoto, Saori Fukuda, Masanori Kugita, Riona Hatazawa, Chitose Koyama, Kazuhiko Katayama, Takayuki Murata, Koki Taniguchi

研究成果: Article

抄録

The generation of recombinant group A rotaviruses (RVAs) entirely from cloned cDNAs has been described only for a single animal RVA strain, simian SA11-L2. We recently developed an optimized RVA reverse genetics system based on only RVA cDNAs (11-plasmid system), in which the concentration of cDNA plasmids containing the NSP2 and NSP5 genes is 3- or 5-fold increased in relation to that of the other plasmids. Based on this approach, we generated a recombinant human RVA (HuRVA)-based monoreassortant virus containing the VP4 gene of the simian SA11-L2 virus using the 11-plasmid system. In addition to this monoreassortant virus, authentic HuRVA (strain KU) was also generated with the 11-plasmid system with some modifications. Our results demonstrate that the 11-plasmid system involving just RVA cDNAs can be used for the generation of recombinant HuRVA and recombinant HuRVA-based reassortant viruses. IMPORTANCE Human group A rotavirus (HuRVA) is a leading pathogen causing severe diarrhea in young children worldwide. In this paper, we describe the generation of recombinant HuRVA (strain KU) from only 11 cloned cDNAs encoding the HuRVA genome by reverse genetics. The growth properties of the recombinant HuRVA were similar to those of the parental RVA, providing a powerful tool for better understanding of HuRVA replication and pathogenesis. Furthermore, the ability to manipulate the genome of HuRVAs “to order” will be useful for next-generation vaccine production for this medically important virus and for the engineering of clinical vectors expressing any foreign genes.

元の言語English
記事番号Y
ジャーナルJournal of Virology
93
発行部数8
DOI
出版物ステータスPublished - 01-01-2019

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Rotavirus
Complementary DNA
Genome
genome
plasmids
Plasmids
Viruses
Reverse Genetics
viruses
Reassortant Viruses
Biomedical Engineering
Genes
vertebrate viruses
genes
Human Genome
Diarrhea
diarrhea
engineering
Vaccines
pathogenesis

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

これを引用

Komoto, Satoshi ; Fukuda, Saori ; Kugita, Masanori ; Hatazawa, Riona ; Koyama, Chitose ; Katayama, Kazuhiko ; Murata, Takayuki ; Taniguchi, Koki. / Generation of infectious recombinant human rotaviruses from just 11 cloned cDNAs encoding the rotavirus genome. :: Journal of Virology. 2019 ; 巻 93, 番号 8.
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abstract = "The generation of recombinant group A rotaviruses (RVAs) entirely from cloned cDNAs has been described only for a single animal RVA strain, simian SA11-L2. We recently developed an optimized RVA reverse genetics system based on only RVA cDNAs (11-plasmid system), in which the concentration of cDNA plasmids containing the NSP2 and NSP5 genes is 3- or 5-fold increased in relation to that of the other plasmids. Based on this approach, we generated a recombinant human RVA (HuRVA)-based monoreassortant virus containing the VP4 gene of the simian SA11-L2 virus using the 11-plasmid system. In addition to this monoreassortant virus, authentic HuRVA (strain KU) was also generated with the 11-plasmid system with some modifications. Our results demonstrate that the 11-plasmid system involving just RVA cDNAs can be used for the generation of recombinant HuRVA and recombinant HuRVA-based reassortant viruses. IMPORTANCE Human group A rotavirus (HuRVA) is a leading pathogen causing severe diarrhea in young children worldwide. In this paper, we describe the generation of recombinant HuRVA (strain KU) from only 11 cloned cDNAs encoding the HuRVA genome by reverse genetics. The growth properties of the recombinant HuRVA were similar to those of the parental RVA, providing a powerful tool for better understanding of HuRVA replication and pathogenesis. Furthermore, the ability to manipulate the genome of HuRVAs “to order” will be useful for next-generation vaccine production for this medically important virus and for the engineering of clinical vectors expressing any foreign genes.",
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Generation of infectious recombinant human rotaviruses from just 11 cloned cDNAs encoding the rotavirus genome. / Komoto, Satoshi; Fukuda, Saori; Kugita, Masanori; Hatazawa, Riona; Koyama, Chitose; Katayama, Kazuhiko; Murata, Takayuki; Taniguchi, Koki.

:: Journal of Virology, 巻 93, 番号 8, Y, 01.01.2019.

研究成果: Article

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