Generation of mouse pluripotent stem cell-derived proliferating myeloid cells as an unlimited source of functional antigen-presenting cells

Rong Zhang, Tian Yi Liu, Satoru Senju, Miwa Haruta, Narumi Hirosawa, Motoharu Suzuki, Minako Tatsumi, Norihiro Ueda, Hiroyuki Maki, Ryusuke Nakatsuka, Yoshikazu Matsuoka, Yutaka Sasaki, Shinobu Tsuzuki, Hayao Nakanishi, Ryoko Araki, Masumi Abe, Yoshiki Akatsuka, Yasushi Sakamoto, Yoshiaki Sonoda, Yasuharu NishimuraKiyotaka Kuzushima, Yasushi Uemura

研究成果: Article

10 被引用数 (Scopus)

抄録

The use of dendritic cells (DC) to prime tumor-associated antigen-specific T-cell responses provides a promising approach to cancer immunotherapy. Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) can differentiate into functional DCs, thus providing an unlimited source of DCs. However, thepreviously established methods of generating practical volumes of DCs from pluripotent stem cells (PSC) require a large number of PSCs at the start of the differentiation culture. In this study, we generated mouse proliferating myeloid cells (pMC) as a source of antigen-presenting cells (APC) using lentivirus-mediated transduction of the c-Myc gene into mouse PSC-derived myeloid cells. The pMCs could propagate almost indefinitely in a cytokine-dependent manner, while retaining their potential to differentiate into functional APCs. After treatment with IL4 plus GM-CSF, the pMCs showed impaired proliferation and differentiated into immature DC-like cells (pMC-DC) expressing low levels of major histocompatibility complex (MHC)-I, MHC-II, CD40, CD80, and CD86. In addition, exposure to maturation stimuli induced the production of TNFa and IL12p70, and enhanced the expression of MHC-II, CD40, and CD86, which is thus suggestive of typical DC maturation. Similar to bone marrow-derived DCs, they stimulated a primary mixed lymphocyte reaction. Furthermore, the in vivo transfer of pMC-DCs pulsed with H-2Kb-restricted OVA257-264 peptide primed OVA-specific cytotoxic T cells and elicited protection in mice against challenge with OVA-expressing melanoma. Overall, myeloid cells exhibiting cytokine-dependent proliferation and DC-like differentiation may be used to address issues associated with the preparation of DCs.

本文言語English
ページ(範囲)668-677
ページ数10
ジャーナルCancer Immunology Research
3
6
DOI
出版ステータスPublished - 06-2015

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cancer Research

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