@article{68cb21dc600e404d8ed27a3294d35267,
title = "Generation of recombinant rotaviruses expressing fluorescent proteins by using an optimized reverse genetics system",
abstract = "An entirely plasmid-based reverse genetics system for rotaviruses was established very recently. We improved the reverse genetics system to generate recombinant rotavirus by transfecting only 11 cDNA plasmids for its 11 gene segments under the condition of increasing the ratio of the cDNA plasmids for NSP2 and NSP5 genes. Utilizing this highly efficient system, we then engineered infectious recombinant rotaviruses expressing bioluminescent (NanoLuc luciferase) and fluorescent (enhanced green fluorescent protein [EGFP] and mCherry) reporters. These recombinant rotaviruses expressing reporters remained genetically stable during serial passages. Our reverse genetics approach and recombinant rotaviruses carrying reporter genes will be great additions to the tool kit for studying the molecular virology of rotavirus and for developing future next-generation vaccines and expression vectors.",
author = "Satoshi Komoto and Saori Fukuda and Tomihiko Ide and Naoto Ito and Makoto Sugiyama and Tetsushi Yoshikawa and Takayuki Murata and Koki Taniguchi",
note = "Funding Information: We thank Naoe Kotomura, Satoru Ishihara, Naoki Yahata, Akiko Kondo, Kentaro Tsuka-moto, and Naoki Yamamoto (Fujita Health University, Aichi) for their technical advice. This study was supported in part by the MEXT-Supported Program for the Research Funding Information: Program on Emerging and Re-emerging Infectious Diseases of the Japan Agency for Medical Research and Development, AMED (K.T. and S.K.), and JSPS KAKENHI grant number 15K08505 (S.K.). We have no conflicts of interests to declare. Funding Information: We thank Naoe Kotomura, Satoru Ishihara, Naoki Yahata, Akiko Kondo, Kentaro Tsukamoto, and Naoki Yamamoto (Fujita Health University, Aichi) for their technical advice. This study was supported in part by the MEXT-Supported Program for the Research Program on Emerging and Re-emerging Infectious Diseases of the Japan Agency for Medical Research and Development, AMED (K.T. and S.K.), and JSPS KAKENHI grant number 15K08505 (S.K.). We have no conflicts of interests to declare. Publisher Copyright: {\textcopyright} 2018 American Society for Microbiology.",
year = "2018",
month = jul,
day = "1",
doi = "10.1128/JVI.00588-18",
language = "English",
volume = "92",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "13",
}