TY - JOUR
T1 - Genistein inhibits Ca2+ influx by extracellular ATP in PC12 pheochromocytoma cells
AU - Kozawa, O.
AU - Shinoda, J.
AU - Suzuki, A.
PY - 1995
Y1 - 1995
N2 - We previously showed that extracellular ATP-induced Ca2+ influx is inhibited by self-activated protein kinase C in rat PC12 pheochromocytoma cells. In the present study, we examined whether tyrosine kinase is involved in the ATP-induced Ca2+ influx in PC12 cells. Genistein, an inhibitor of protein tyrosine kinases, which by itself had little effect on 45Ca2+ influx, significantly suppressed the ATP-induced 45Ca2+ influx in a dose-dependent manner in the range between 1 and 30 μg/ml. Tyrphostin, another inhibitor of tyrosine kinases chemically distinct from genistein, also inhibited the 45Ca2+ influx. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, markedly enhanced the ATP-induced 45Ca2+ influx. These results suggest that tyrosine kinase regulates Ca2+ influx induced by extracellular ATP in PC12 pheochromocytoma cells.
AB - We previously showed that extracellular ATP-induced Ca2+ influx is inhibited by self-activated protein kinase C in rat PC12 pheochromocytoma cells. In the present study, we examined whether tyrosine kinase is involved in the ATP-induced Ca2+ influx in PC12 cells. Genistein, an inhibitor of protein tyrosine kinases, which by itself had little effect on 45Ca2+ influx, significantly suppressed the ATP-induced 45Ca2+ influx in a dose-dependent manner in the range between 1 and 30 μg/ml. Tyrphostin, another inhibitor of tyrosine kinases chemically distinct from genistein, also inhibited the 45Ca2+ influx. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, markedly enhanced the ATP-induced 45Ca2+ influx. These results suggest that tyrosine kinase regulates Ca2+ influx induced by extracellular ATP in PC12 pheochromocytoma cells.
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U2 - 10.1055/s-2007-979957
DO - 10.1055/s-2007-979957
M3 - Article
C2 - 7557837
AN - SCOPUS:0028978602
VL - 27
SP - 272
EP - 274
JO - Hormone and Metabolic Research
JF - Hormone and Metabolic Research
SN - 0018-5043
IS - 6
ER -