抄録
We previously showed that extracellular ATP-induced Ca2+ influx is inhibited by self-activated protein kinase C in rat PC12 pheochromocytoma cells. In the present study, we examined whether tyrosine kinase is involved in the ATP-induced Ca2+ influx in PC12 cells. Genistein, an inhibitor of protein tyrosine kinases, which by itself had little effect on 45Ca2+ influx, significantly suppressed the ATP-induced 45Ca2+ influx in a dose-dependent manner in the range between 1 and 30 μg/ml. Tyrphostin, another inhibitor of tyrosine kinases chemically distinct from genistein, also inhibited the 45Ca2+ influx. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, markedly enhanced the ATP-induced 45Ca2+ influx. These results suggest that tyrosine kinase regulates Ca2+ influx induced by extracellular ATP in PC12 pheochromocytoma cells.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 272-274 |
| ページ数 | 3 |
| ジャーナル | Hormone and Metabolic Research |
| 巻 | 27 |
| 発行部数 | 6 |
| 出版物ステータス | Published - 01-01-1995 |
| 外部発表 | Yes |
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All Science Journal Classification (ASJC) codes
- Endocrinology, Diabetes and Metabolism
- Biochemistry
- Endocrinology
- Clinical Biochemistry
- Biochemistry, medical
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Genistein inhibits Ca2+ influx by extracellular ATP in PC12 pheochromocytoma cells. / Kozawa, O.; Shinoda, J.; Suzuki, Atsushi.
：: Hormone and Metabolic Research, 巻 27, 番号 6, 01.01.1995, p. 272-274.研究成果: Article
TY - JOUR
T1 - Genistein inhibits Ca2+ influx by extracellular ATP in PC12 pheochromocytoma cells
AU - Kozawa, O.
AU - Shinoda, J.
AU - Suzuki, Atsushi
PY - 1995/1/1
Y1 - 1995/1/1
N2 - We previously showed that extracellular ATP-induced Ca2+ influx is inhibited by self-activated protein kinase C in rat PC12 pheochromocytoma cells. In the present study, we examined whether tyrosine kinase is involved in the ATP-induced Ca2+ influx in PC12 cells. Genistein, an inhibitor of protein tyrosine kinases, which by itself had little effect on 45Ca2+ influx, significantly suppressed the ATP-induced 45Ca2+ influx in a dose-dependent manner in the range between 1 and 30 μg/ml. Tyrphostin, another inhibitor of tyrosine kinases chemically distinct from genistein, also inhibited the 45Ca2+ influx. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, markedly enhanced the ATP-induced 45Ca2+ influx. These results suggest that tyrosine kinase regulates Ca2+ influx induced by extracellular ATP in PC12 pheochromocytoma cells.
AB - We previously showed that extracellular ATP-induced Ca2+ influx is inhibited by self-activated protein kinase C in rat PC12 pheochromocytoma cells. In the present study, we examined whether tyrosine kinase is involved in the ATP-induced Ca2+ influx in PC12 cells. Genistein, an inhibitor of protein tyrosine kinases, which by itself had little effect on 45Ca2+ influx, significantly suppressed the ATP-induced 45Ca2+ influx in a dose-dependent manner in the range between 1 and 30 μg/ml. Tyrphostin, another inhibitor of tyrosine kinases chemically distinct from genistein, also inhibited the 45Ca2+ influx. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, markedly enhanced the ATP-induced 45Ca2+ influx. These results suggest that tyrosine kinase regulates Ca2+ influx induced by extracellular ATP in PC12 pheochromocytoma cells.
UR - http://www.scopus.com/inward/record.url?scp=0028978602&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028978602&partnerID=8YFLogxK
M3 - Article
C2 - 7557837
AN - SCOPUS:0028978602
VL - 27
SP - 272
EP - 274
JO - Hormone and Metabolic Research
JF - Hormone and Metabolic Research
SN - 0018-5043
IS - 6
ER -