Germ cell survival through carbohydrate-mediated interaction with Sertoli cells

Tomoya O. Akama, Hiroaki Nakagawa, Kazuhiro Sugihara, Sonoko Narisawa, Chikara Ohyama, Shin Ichiro Nishimura, Deborah A. O'Brien, Kelley W. Moremen, José Luis Millán, Michiko N. Fukuda

研究成果: Article査読

122 被引用数 (Scopus)

抄録

Spermatogenesis is a precisely regulated process in which the germ cells closely interact with Sertoli cells. The molecular basis of this cell-cell adhesion is unknown. Here, we demonstrate that targeted disruption of Man2a2, a gene encoding α-mannosidase llx (MX), an enzyme that forms intermediate asparagine-linked carbohydrates (N-glycans), results in Man2a2 null males that are largely infertile. The Man2a2 null spermatogenic cells fail to adhere to Sertoli cells and are prematurely released from the testis to epididymis. We identified an N-glycan structure that plays a key role in germ cell-Sertoli cell adhesion and showed that a specific carbohydrate was required for spermatogenesis.

本文言語English
ページ(範囲)124-127
ページ数4
ジャーナルScience
295
5552
DOI
出版ステータスPublished - 04-01-2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • 一般

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