Girdin and its phosphorylation dynamically regulate neonatal vascular development and pathological neovascularization in the retina

Takanori Ito, Keiichi Komeima, Tetsuhiro Yasuma, Atsushi Enomoto, Naoya Asai, Masato Asai, Sayoko Iwase, Masahide Takahashi, Hiroko Terasaki

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Vascular endothelial growth factor (VEGF) is recognized as a principal mediator of vessel growth. VEGF regulates various endothelial cellular processes, including cell migration, proliferation, and survival, through the serine threonine protein kinase Akt. The Akt substrate girdin, an actin-binding protein, is known to regulate VEGF-mediated postnatal angiogenesis. However, the role of girdin and its phosphorylation in neonatal retinal vascular development and ocular pathological neovascularization in vivo has not been elucidated. In the present study, therefore, we investigated these processes using Girdin +/- mice lacking one copy of the girdin gene and girdin S1416A knockin (Girdin-KISA/SA) mice in which the phosphorylation site of girdin is completely disrupted. We used three mouse models of pathological ocular neovascularization: oxygen-induced retinopathy (a mouse model of ischemic retinopathies), laser-induced choroidal neovascularization, and a human VEGF transgenic mouse that overexpresses human VEGF specifically in photoreceptor cells and generates pathological neovascularization in the retina. Neonatal vascular development was delayed and pathological neovascularization was decreased in both Girdin+/- mice and Girdin-KISA/SA mice. These results demonstrate that girdin and its phosphorylation play an important role in neonatal vascular development and in pathological neovascularization in the retina.

本文言語English
ページ(範囲)586-596
ページ数11
ジャーナルAmerican Journal of Pathology
182
2
DOI
出版ステータスPublished - 02-2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 病理学および法医学

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