Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells

Yuichi Hirose; Yoshinori Imai; Kazuyuki Nakajima; Nagisa Takemoto; Shigeo Toya; Shinichi Kohsaka

doi:10.1006/bbrc.1994.1074

Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells

Yuichi Hirose, Yoshinori Imai, Kazuyuki Nakajima, Nagisa Takemoto, Shigeo Toya, Shinichi Kohsaka

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

22 被引用数 (Scopus)

抄録

The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons.

本文言語	英語
ページ（範囲）	504-509
ページ数	6
ジャーナル	Biochemical and Biophysical Research Communications
巻	198
号	2
DOI	https://doi.org/10.1006/bbrc.1994.1074
出版ステータス	出版済み - 31-01-1994
外部発表	はい

All Science Journal Classification (ASJC) codes

生物理学
生化学
分子生物学
細胞生物学

文献へのアクセス

10.1006/bbrc.1994.1074

他のファイルとリンク

引用スタイル

@article{6bc7238cc284420d8f61bb00be1b9238,

title = "Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells",

abstract = "The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons.",

author = "Yuichi Hirose and Yoshinori Imai and Kazuyuki Nakajima and Nagisa Takemoto and Shigeo Toya and Shinichi Kohsaka",

year = "1994",

month = jan,

day = "31",

doi = "10.1006/bbrc.1994.1074",

language = "English",

volume = "198",

pages = "504--509",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells

AU - Hirose, Yuichi

AU - Imai, Yoshinori

AU - Nakajima, Kazuyuki

AU - Takemoto, Nagisa

AU - Toya, Shigeo

AU - Kohsaka, Shinichi

PY - 1994/1/31

Y1 - 1994/1/31

N2 - The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons.

AB - The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons.

UR - http://www.scopus.com/inward/record.url?scp=0028362369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028362369&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1994.1074

DO - 10.1006/bbrc.1994.1074

M3 - Article

C2 - 8297361

AN - SCOPUS:0028362369

SN - 0006-291X

VL - 198

SP - 504

EP - 509

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells

抄録

All Science Journal Classification (ASJC) codes

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル