抄録
Because of the worldwide shortage of renal grafts, kidney transplantation (KTx) from donors after cardiac death (DCD) is an alternative way to obtain KTx from brain-dead donors. Although the prognosis of DCD KTx is gradually improving, the graft often undergoes delayed graft function (DGF), rendering the control of DGF essential for post-KTx patient care. In an attempt to characterize etiology of DGF, genome-wide gene expression profiling was performed using renal biopsy samples performed at 1 h after KTx from DCD and the data were compared with those of KTx from living donors (LD). A total of 526 genes were differentially expressed between them. Genes involved in acute inflammation were activated, while metabolic pathways were consistently downregulated in DCD. These findings imply the inferior performance of the DCD grafts relative to LD grafts. Several genes were identified where the expression levels were correlated well with parameters indicating short- and long-term prognosis of the DCD patients. In addition, several genes encoding secretory proteins were identified that might reflect the performance of the graft and be potential noninvasive biomarkers. These data provide a good source for candidates of biomarkers that are potentially useful for the control of DGF.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 647-656 |
| ページ数 | 10 |
| ジャーナル | Cell Transplantation |
| 巻 | 18 |
| 発行部数 | 5-6 |
| DOI | |
| 出版物ステータス | Published - 2009 |
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All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- Cell Biology
- Transplantation
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Global expression profiles in 1-hour biopsy specimens of human kidney transplantation from donors after cardiac death. / Kusaka, Mamoru; Kuroyanagi, Yoko; Mori, Terumi; Nagaoka, Kayuri; Sasaki, Hitomi; Maruyama, Takahiro; Hayakawa, Kunihiro; Shiroki, Ryoichi; Kurahashi, Hiroki; Hoshinaga, Kiyotaka.
：: Cell Transplantation, 巻 18, 番号 5-6, 2009, p. 647-656.研究成果: Article
TY - JOUR
T1 - Global expression profiles in 1-hour biopsy specimens of human kidney transplantation from donors after cardiac death
AU - Kusaka, Mamoru
AU - Kuroyanagi, Yoko
AU - Mori, Terumi
AU - Nagaoka, Kayuri
AU - Sasaki, Hitomi
AU - Maruyama, Takahiro
AU - Hayakawa, Kunihiro
AU - Shiroki, Ryoichi
AU - Kurahashi, Hiroki
AU - Hoshinaga, Kiyotaka
PY - 2009
Y1 - 2009
N2 - Because of the worldwide shortage of renal grafts, kidney transplantation (KTx) from donors after cardiac death (DCD) is an alternative way to obtain KTx from brain-dead donors. Although the prognosis of DCD KTx is gradually improving, the graft often undergoes delayed graft function (DGF), rendering the control of DGF essential for post-KTx patient care. In an attempt to characterize etiology of DGF, genome-wide gene expression profiling was performed using renal biopsy samples performed at 1 h after KTx from DCD and the data were compared with those of KTx from living donors (LD). A total of 526 genes were differentially expressed between them. Genes involved in acute inflammation were activated, while metabolic pathways were consistently downregulated in DCD. These findings imply the inferior performance of the DCD grafts relative to LD grafts. Several genes were identified where the expression levels were correlated well with parameters indicating short- and long-term prognosis of the DCD patients. In addition, several genes encoding secretory proteins were identified that might reflect the performance of the graft and be potential noninvasive biomarkers. These data provide a good source for candidates of biomarkers that are potentially useful for the control of DGF.
AB - Because of the worldwide shortage of renal grafts, kidney transplantation (KTx) from donors after cardiac death (DCD) is an alternative way to obtain KTx from brain-dead donors. Although the prognosis of DCD KTx is gradually improving, the graft often undergoes delayed graft function (DGF), rendering the control of DGF essential for post-KTx patient care. In an attempt to characterize etiology of DGF, genome-wide gene expression profiling was performed using renal biopsy samples performed at 1 h after KTx from DCD and the data were compared with those of KTx from living donors (LD). A total of 526 genes were differentially expressed between them. Genes involved in acute inflammation were activated, while metabolic pathways were consistently downregulated in DCD. These findings imply the inferior performance of the DCD grafts relative to LD grafts. Several genes were identified where the expression levels were correlated well with parameters indicating short- and long-term prognosis of the DCD patients. In addition, several genes encoding secretory proteins were identified that might reflect the performance of the graft and be potential noninvasive biomarkers. These data provide a good source for candidates of biomarkers that are potentially useful for the control of DGF.
UR - http://www.scopus.com/inward/record.url?scp=70349235405&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349235405&partnerID=8YFLogxK
U2 - 10.1177/096368970901805-621
DO - 10.1177/096368970901805-621
M3 - Article
C2 - 19775527
AN - SCOPUS:70349235405
VL - 18
SP - 647
EP - 656
JO - Cell Transplantation
JF - Cell Transplantation
SN - 0963-6897
IS - 5-6
ER -