GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction

Ikuko Miyajima, Masato Nakafuku, Naoki Nakayama, Charles Brenner, Atsushi Miyajima, Kozo Kaibuchi, Ken ichi Arai, Yoshito Kaziro, Kunihiro Matsumoto

研究成果: Article査読

208 被引用数 (Scopus)

抄録

GPA1 protein of Saccharomyces cerevisiae is homologous to the α subunit of mammalian G protein. GPA1 transcript was found in haploid cells but was not detected in diploid cells. Disruption of GPA1 resulted in a haploid-specific lethal phenotype, indicating that GPA1 is a haploid-specific essential gene for cell growth. Upon regulation of expression of GPA1 by the galactose-inducible GAL1 promoter, the loss of GPA1 function was found to lead to cell-cycle arrest at the late G1 phase. Mutants that suppress the lethality of the gpa1::HIS3 mutation showed a sterile phenotype that was not cell-type-specific. These results suggest that GPA1 protein may control the signal for mating-factor-mediated cell-cycle arrest.

本文言語English
ページ(範囲)1011-1019
ページ数9
ジャーナルCell
50
7
DOI
出版ステータスPublished - 25-09-1987
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)

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