Growth‐Promoting Action of Adenosine‐Containing Dinucleotide on Neuroblastoma Cells: Detection of Adenosine‐Cytidine Dinucleotide (ApCp) in Neurofibroma (NF1) Extracts

Taeko Hotta, Kiyofumi Asai, Naohito Takeda, Hideo Yoshizumi, Akira Tatematsu, Keiko Nakanishi, Yaman Z. Eksioglu, Ichiro Isobe, Taiji Kato

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Abstract: Neurofibroma type 1 tissue was investigated for the presence of growth‐promoting activity on human neuroblastoma cells. The activity was isolated by gel filtration and reversed‐phase column chromatographs from neurofibroma type 1 extracts. An adenosine‐containing dinucleotide (adenylyl(3′‐5′)cytidine‐3′‐phosphate) was identified as one of the major components of the activities by its enzymatic fragmentation and liquid chromatography/mass spectrometry. Synthetic adenosine‐containing dinucleotide derivatives such as cytidyl(3′‐5′)adenosine, cytidyl(2′‐5′)adenosine, adenylyl(3′‐5′)cytidine, and adenylyl(2′‐5′)cytidine showed a similar action. Cytidyl(3′‐5′)adenosine, cytidyl(2′‐5′)adenosine, and adenylyl(2′‐5′)cytidine, which are able to release a free adenosine through enzymatic hydrolysis, in particular elicited a strong activity corresponding to that of adenosine with the highest action. These results suggest that neuroblastoma cells are able to use adenosine‐containing dinucleotides as well as mononucleotides for their survival and proliferation.

本文言語English
ページ(範囲)1430-1437
ページ数8
ジャーナルJournal of neurochemistry
61
4
DOI
出版ステータスPublished - 10-1993
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 細胞および分子神経科学

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