TY - JOUR
T1 - Heart-derived collagen promotes maturation of engineered heart tissue
AU - Tani, Hidenori
AU - Kobayashi, Eiji
AU - Yagi, Shinomi
AU - Tanaka, Keisuke
AU - Kameda-Haga, Kotaro
AU - Shibata, Shinsuke
AU - Moritoki, Nobuko
AU - Takatsuna, Kaworu
AU - Moriwaki, Taijun
AU - Sekine, Otoya
AU - Umei, Tomohiko C.
AU - Morita, Yuika
AU - Soma, Yusuke
AU - Kishino, Yoshikazu
AU - Kanazawa, Hideaki
AU - Fujita, Jun
AU - Hattori, Shunji
AU - Fukuda, Keiichi
AU - Tohyama, Shugo
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2023/8
Y1 - 2023/8
N2 - Although the extracellular matrix (ECM) plays essential roles in heart tissue engineering, the optimal ECM components for heart tissue organization have not previously been elucidated. Here, we focused on the main ECM component, fibrillar collagen, and analyzed the effects of collagens on heart tissue engineering, by comparing the use of porcine heart-derived collagen and other organ-derived collagens in generating engineered heart tissue (EHT). We demonstrate that heart-derived collagen induces better contraction and relaxation of human induced pluripotent stem cell-derived EHT (hiPSC-EHT) and that hiPSC-EHT with heart-derived collagen exhibit more mature profiles than those with collagens from other organs. Further, we found that collagen fibril formation and gel stiffness influence the contraction, relaxation, and maturation of hiPSC-EHT, suggesting the importance of collagen types III and type V, which are relatively abundant in the heart. Thus, we demonstrate the effectiveness of organ-specific collagens in tissue engineering and drug discovery.
AB - Although the extracellular matrix (ECM) plays essential roles in heart tissue engineering, the optimal ECM components for heart tissue organization have not previously been elucidated. Here, we focused on the main ECM component, fibrillar collagen, and analyzed the effects of collagens on heart tissue engineering, by comparing the use of porcine heart-derived collagen and other organ-derived collagens in generating engineered heart tissue (EHT). We demonstrate that heart-derived collagen induces better contraction and relaxation of human induced pluripotent stem cell-derived EHT (hiPSC-EHT) and that hiPSC-EHT with heart-derived collagen exhibit more mature profiles than those with collagens from other organs. Further, we found that collagen fibril formation and gel stiffness influence the contraction, relaxation, and maturation of hiPSC-EHT, suggesting the importance of collagen types III and type V, which are relatively abundant in the heart. Thus, we demonstrate the effectiveness of organ-specific collagens in tissue engineering and drug discovery.
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U2 - 10.1016/j.biomaterials.2023.122174
DO - 10.1016/j.biomaterials.2023.122174
M3 - Article
C2 - 37285642
AN - SCOPUS:85160816399
SN - 0142-9612
VL - 299
JO - Biomaterials
JF - Biomaterials
M1 - 122174
ER -