Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method

Ryota Suzuki, Masaru Ihira, Yoshihiko Enomoto, Hiroaki Yano, Fumio Maruyama, Nobuhiko Emi, Yoshizo Asano, Tetsushi Yoshikawa

研究成果: Comment/debate

17 引用 (Scopus)

抄録

A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of350 samples (29.4%) by the original CMV LAMP procedure and 148 of350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.

元の言語English
ページ(範囲)466-470
ページ数5
ジャーナルMicrobiology and Immunology
54
発行部数8
DOI
出版物ステータスPublished - 01-01-2010

Fingerprint

Cytomegalovirus
Hot Temperature
Sensitivity and Specificity
DNA
MEC protocol 1

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

これを引用

Suzuki, Ryota ; Ihira, Masaru ; Enomoto, Yoshihiko ; Yano, Hiroaki ; Maruyama, Fumio ; Emi, Nobuhiko ; Asano, Yoshizo ; Yoshikawa, Tetsushi. / Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method. :: Microbiology and Immunology. 2010 ; 巻 54, 番号 8. pp. 466-470.
@article{e1b8d071b97f4decb9f4ce6b91c74d06,
title = "Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method",
abstract = "A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of350 samples (29.4{\%}) by the original CMV LAMP procedure and 148 of350 samples (42.3{\%}) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9{\%}, 77.7{\%}, 62.2{\%}, and 96.5{\%}, respectively.",
author = "Ryota Suzuki and Masaru Ihira and Yoshihiko Enomoto and Hiroaki Yano and Fumio Maruyama and Nobuhiko Emi and Yoshizo Asano and Tetsushi Yoshikawa",
year = "2010",
month = "1",
day = "1",
doi = "10.1111/j.1348-0421.2010.00236.x",
language = "English",
volume = "54",
pages = "466--470",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "8",

}

Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method. / Suzuki, Ryota; Ihira, Masaru; Enomoto, Yoshihiko; Yano, Hiroaki; Maruyama, Fumio; Emi, Nobuhiko; Asano, Yoshizo; Yoshikawa, Tetsushi.

:: Microbiology and Immunology, 巻 54, 番号 8, 01.01.2010, p. 466-470.

研究成果: Comment/debate

TY - JOUR

T1 - Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method

AU - Suzuki, Ryota

AU - Ihira, Masaru

AU - Enomoto, Yoshihiko

AU - Yano, Hiroaki

AU - Maruyama, Fumio

AU - Emi, Nobuhiko

AU - Asano, Yoshizo

AU - Yoshikawa, Tetsushi

PY - 2010/1/1

Y1 - 2010/1/1

N2 - A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of350 samples (29.4%) by the original CMV LAMP procedure and 148 of350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.

AB - A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of350 samples (29.4%) by the original CMV LAMP procedure and 148 of350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.

UR - http://www.scopus.com/inward/record.url?scp=77955000280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955000280&partnerID=8YFLogxK

U2 - 10.1111/j.1348-0421.2010.00236.x

DO - 10.1111/j.1348-0421.2010.00236.x

M3 - Comment/debate

C2 - 20646211

AN - SCOPUS:77955000280

VL - 54

SP - 466

EP - 470

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 8

ER -