TY - JOUR
T1 - Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method
AU - Suzuki, Ryota
AU - Ihira, Masaru
AU - Enomoto, Yoshihiko
AU - Yano, Hiroaki
AU - Maruyama, Fumio
AU - Emi, Nobuhiko
AU - Asano, Yoshizo
AU - Yoshikawa, Tetsushi
N1 - Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2010/8
Y1 - 2010/8
N2 - A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of350 samples (29.4%) by the original CMV LAMP procedure and 148 of350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.
AB - A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of350 samples (29.4%) by the original CMV LAMP procedure and 148 of350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.
UR - http://www.scopus.com/inward/record.url?scp=77955000280&partnerID=8YFLogxK
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U2 - 10.1111/j.1348-0421.2010.00236.x
DO - 10.1111/j.1348-0421.2010.00236.x
M3 - Comment/debate
C2 - 20646211
AN - SCOPUS:77955000280
SN - 0385-5600
VL - 54
SP - 466
EP - 470
JO - MICROBIOLOGY and IMMUNOLOGY
JF - MICROBIOLOGY and IMMUNOLOGY
IS - 8
ER -