Hepatitis B virus genotype assignment using restriction fragment length polymorphism patterns

Masashi Mizokami, Tatsunori Nakano, Etsuro Orito, Yasuhito Tanaka, Hiroshi Sakugawa, Motokazu Mukaide, Betty H. Robertson

研究成果: Article

228 引用 (Scopus)

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Hepatitis B virus (HBV) is classified into genotypes A-F, which is important for clinical and etiological investigations. To establish a simple genotyping method, 68 full-genomic sequences and 106 S gene sequences were analyzed by the molecular evolutionary method. HBV genotyping with the S gene sequence is consistent with genetic analysis using the full-genomic sequence. After alignment of the S sequences, genotype specific regions are identified and digested by the restriction enzymes, HphI, NciI, AlwI, EarI, and NlaIV. This HBV genotyping system using restriction fragment length polymorphism (RFLP) was confirmed to be correct when the PCR products of the S gene in 23 isolates collected from various countries were digested with this method. A restriction site for EarI in genotype B was absent in spite of its presence in all the other genotypes and genotype C has no restriction site for AlwI. Only genotype E is digested with NciI, while only genotype F has a restriction site for HphI. Genotype A can be distinguished by a single restriction enzyme site for NlaIV, while genotype D digestion with this enzyme results in two products that migrates at 265 and 186 bp. This simple and accurate HBV genotyping system using RFLP is considered to be useful for research on HBV. Copyright (C) 1999 Federation of European Biochemical Societies.

元の言語English
ページ(範囲)66-71
ページ数6
ジャーナルFEBS Letters
450
発行部数1-2
DOI
出版物ステータスPublished - 30-04-1999
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • これを引用

    Mizokami, M., Nakano, T., Orito, E., Tanaka, Y., Sakugawa, H., Mukaide, M., & Robertson, B. H. (1999). Hepatitis B virus genotype assignment using restriction fragment length polymorphism patterns. FEBS Letters, 450(1-2), 66-71. https://doi.org/10.1016/S0014-5793(99)00471-8