Hepatocyte transplantation in rats with decompensated cirrhosis

Naoya Kobayashi, Masahiro Ito, Junta Nakamura, Jin Cai, Chen Gao, James M. Hammel, Ira J. Fox

研究成果: Article査読

126 被引用数 (Scopus)

抄録

Hepatocyte transplantation improves the survival of laboratory animals with experimentally induced acute liver failure and the physiological abnormalities associated with liver-based metabolic deficiencies. The role of hepatocyte transplantation in treating decompensated liver cirrhosis, however, has not been studied in depth. To address this issue, cirrhosis was induced using phenobarbital and carbon tetrachloride (CCL4) and animals were studied only when evidence of liver failure did not improve when eel4 was held for 4 weeks. Animals received intrasplenic transplantation of syngeneic rat hepatocytes (G1); intraperitoneal transplantation of syngeneic rat hepatocytes (G2); intraperitoneal transplantation of a cellular homogenate of syngeneic rat hepatocytes (G3); intraperitoneal transplantation of syngeneic rat bone marrow cells (G4); or intrasplenic injection of Dulbecco's modified Eagle medium (DMEM) (G5). After transplantation, body weight and serum albumin levels deteriorated over time in all control (G2-G5) animals but did not deteriorate in animals receiving intrasplenic hepatocyte transplantation (G1) (P < .01). Prothrombin time (PT), total bilirubin, serum ammonia, and hepatic encephalopathy score were also significantly improved toward normal in animals receiving intrasplenic hepatocyte transplantation (P < .01). More importantly, survival was prolonged after a single infusion of hepatocytes and a second infusion prolonged survival from 15 to 128 days (P < .01). Thus, hepatocyte transplantation can improve liver function and prolong the survival of rats with irreversible, decompensated cirrhosis and may be useful in the treatment of cirrhosis in humans.

本文言語English
ページ(範囲)851-857
ページ数7
ジャーナルHepatology
31
4
DOI
出版ステータスPublished - 01-01-2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • Hepatology

フィンガープリント 「Hepatocyte transplantation in rats with decompensated cirrhosis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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