TY - JOUR
T1 - High Levels of Dietary Lard or Sucrose May Aggravate Lysosomal Renal Injury in Non-Obese, Streptozotocin-Injected CD-1 Mice Provided Isocaloric Diets
AU - Yoshimura, Aya
AU - Yamaguchi, Tamio
AU - Kugita, Masanori
AU - Kumamoto, Kanako
AU - Shiogama, Kazuya
AU - Ogitsu, Naomichi
AU - Yoneda, Misao
AU - Miura, Toshihiro
AU - Nagamura, Yoichi
AU - Nagao, Shizuko
N1 - Funding Information:
This work was supported by the Grant-in-Aid for Scientific Research, The Ministry of Education, Culture, Sports, Science and Technology [grant number 15K08435].
Publisher Copyright:
© 2021, Center for Academic Publications Japan. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Summary Daily fat and sugar intake has increased in Japan, while total energy intake has decreased. However, the number of type 2 diabetes mellitus patients has increased, and this often causes renal injury characterized by autophagic vacuoles. Although many studies with comparisons of high fat or sugar versus a normal macronutrient balanced diet have been reported, there are few studies that equalized calorie intake and body weights. In the current study, AIN93M diets (CONT group) with matching energy content with lard derived high saturated fat (LARD group), soybean oil derived unsaturated fat (SOY OIL group) and sucrose (SUCROSE group) were provided to compare their effects on renal morphology in streptozotocin-injected CD-1 mice without causing obesity. The number of renal tubular vacuoles was higher in SUCROSE and slightly higher in LARD compared with CONT mice, and was higher in LARD and SUCROSE compared with SOY OIL mice. Most of those vacuoles were LAMP1-positive, a marker of lysosomal autophagy. These results suggest that despite identical energy contents, diets with high sucrose or saturated fat compared to unsaturated fat may aggravate lysosomal renal injury in a non-obese, streptozotocin-induced model of diabetes mellitus.
AB - Summary Daily fat and sugar intake has increased in Japan, while total energy intake has decreased. However, the number of type 2 diabetes mellitus patients has increased, and this often causes renal injury characterized by autophagic vacuoles. Although many studies with comparisons of high fat or sugar versus a normal macronutrient balanced diet have been reported, there are few studies that equalized calorie intake and body weights. In the current study, AIN93M diets (CONT group) with matching energy content with lard derived high saturated fat (LARD group), soybean oil derived unsaturated fat (SOY OIL group) and sucrose (SUCROSE group) were provided to compare their effects on renal morphology in streptozotocin-injected CD-1 mice without causing obesity. The number of renal tubular vacuoles was higher in SUCROSE and slightly higher in LARD compared with CONT mice, and was higher in LARD and SUCROSE compared with SOY OIL mice. Most of those vacuoles were LAMP1-positive, a marker of lysosomal autophagy. These results suggest that despite identical energy contents, diets with high sucrose or saturated fat compared to unsaturated fat may aggravate lysosomal renal injury in a non-obese, streptozotocin-induced model of diabetes mellitus.
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U2 - 10.3177/jnsv.67.243
DO - 10.3177/jnsv.67.243
M3 - Comment/debate
C2 - 34470999
AN - SCOPUS:85115283866
VL - 67
SP - 243
EP - 248
JO - The Journal of vitaminology
JF - The Journal of vitaminology
SN - 0301-4800
IS - 4
ER -