TY - JOUR
T1 - High prevalence of equine-like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand
AU - Tacharoenmuang, Ratana
AU - Komoto, Satoshi
AU - Guntapong, Ratigorn
AU - Upachai, Sompong
AU - Singchai, Phakapun
AU - Ide, Tomihiko
AU - Fukuda, Saori
AU - Ruchusatsawast, Kriangsak
AU - Sriwantana, Busarawan
AU - Tatsumi, Masashi
AU - Motomura, Kazushi
AU - Takeda, Naokazu
AU - Murata, Takayuki
AU - Sangkitporn, Somchai
AU - Taniguchi, Koki
AU - Yoshikawa, Tetsushi
N1 - Funding Information:
We thank the public health staff of the Bhumibol Adulyadej, Phen, Sriwilai, Vachira Phuket, Maesot General, and Phrapokklao Hospitals for their help in collecting specimens and clinical data on the subjects. This study was supported in part by the Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, RCC-ERI (NT), and the laboratory-based surveillance for unidentified and possibly new pathogens affecting public health problem in Thailand, IEIP-EID (BS). This study was also supported by the Research Program on Emerging and Re-emerging Infectious Diseases of the Japan Agency for Medical Research and Development (16fk0108118h1001), AMED (SK), and by RONPAKU (Dissertation PhD) Program, Japan Society for the Promotion of Science (JSPS), Japan (RT).
Publisher Copyright:
© 2019 Wiley Periodicals, Inc.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Group A rotavirus (RVA) is a major cause of acute gastroenteritis in infants and young children worldwide. This study aims to clarify the distribution of G/P types and genetic characteristics of RVAs circulating in Thailand. Between January 2014 and September 2016, 1867 stool specimens were collected from children and adults with acute gastroenteritis in six provinces in Thailand. RVAs were detected in 514/1867 (27.5%) stool specimens. G1P[8] (44.7%) was the most predominant genotype, followed by G3P[8] (33.7%), G2P[4] (11.5%), G8P[8] (7.0%), and G9P[8] (1.3%). Unusual G3P[9] (0.8%), G3P[10] (0.4%), G4P[6] (0.4%), and G10P[14] (0.2%) were also detected at low frequencies. The predominant genotype, G1P[8] (64.4%), in 2014 decreased to 6.1% in 2016. In contrast, the frequency of G3P[8] markedly increased from 5.5% in 2014 to 65.3% in 2015 and 89.8% in 2016. On polyacrylamide gel electrophoresis, most (135/140; 96.4%) of the G3P[8] strains exhibited a short RNA profile. Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine-like G3P[8] strains. On phylogenetic analysis of genome segments of two representative Thai equine-like G3P[8] strains, it was noteworthy that they possessed distinct NSP4 genes, one bovine-like and the other human-like. Thus, we found that characteristic equine-like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand.
AB - Group A rotavirus (RVA) is a major cause of acute gastroenteritis in infants and young children worldwide. This study aims to clarify the distribution of G/P types and genetic characteristics of RVAs circulating in Thailand. Between January 2014 and September 2016, 1867 stool specimens were collected from children and adults with acute gastroenteritis in six provinces in Thailand. RVAs were detected in 514/1867 (27.5%) stool specimens. G1P[8] (44.7%) was the most predominant genotype, followed by G3P[8] (33.7%), G2P[4] (11.5%), G8P[8] (7.0%), and G9P[8] (1.3%). Unusual G3P[9] (0.8%), G3P[10] (0.4%), G4P[6] (0.4%), and G10P[14] (0.2%) were also detected at low frequencies. The predominant genotype, G1P[8] (64.4%), in 2014 decreased to 6.1% in 2016. In contrast, the frequency of G3P[8] markedly increased from 5.5% in 2014 to 65.3% in 2015 and 89.8% in 2016. On polyacrylamide gel electrophoresis, most (135/140; 96.4%) of the G3P[8] strains exhibited a short RNA profile. Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine-like G3P[8] strains. On phylogenetic analysis of genome segments of two representative Thai equine-like G3P[8] strains, it was noteworthy that they possessed distinct NSP4 genes, one bovine-like and the other human-like. Thus, we found that characteristic equine-like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand.
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U2 - 10.1002/jmv.25591
DO - 10.1002/jmv.25591
M3 - Article
C2 - 31498444
AN - SCOPUS:85073971231
VL - 92
SP - 174
EP - 186
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 2
ER -