抄録
Group A rotavirus (RVA) is a major cause of acute gastroenteritis in infants and young children worldwide. This study aims to clarify the distribution of G/P types and genetic characteristics of RVAs circulating in Thailand. Between January 2014 and September 2016, 1867 stool specimens were collected from children and adults with acute gastroenteritis in six provinces in Thailand. RVAs were detected in 514/1867 (27.5%) stool specimens. G1P[8] (44.7%) was the most predominant genotype, followed by G3P[8] (33.7%), G2P[4] (11.5%), G8P[8] (7.0%), and G9P[8] (1.3%). Unusual G3P[9] (0.8%), G3P[10] (0.4%), G4P[6] (0.4%), and G10P[14] (0.2%) were also detected at low frequencies. The predominant genotype, G1P[8] (64.4%), in 2014 decreased to 6.1% in 2016. In contrast, the frequency of G3P[8] markedly increased from 5.5% in 2014 to 65.3% in 2015 and 89.8% in 2016. On polyacrylamide gel electrophoresis, most (135/140; 96.4%) of the G3P[8] strains exhibited a short RNA profile. Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine-like G3P[8] strains. On phylogenetic analysis of genome segments of two representative Thai equine-like G3P[8] strains, it was noteworthy that they possessed distinct NSP4 genes, one bovine-like and the other human-like. Thus, we found that characteristic equine-like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 174-186 |
| ページ数 | 13 |
| ジャーナル | Journal of Medical Virology |
| 巻 | 92 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | Published - 01-02-2020 |
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All Science Journal Classification (ASJC) codes
- Virology
- Infectious Diseases
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High prevalence of equine-like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand. / Tacharoenmuang, Ratana; Komoto, Satoshi; Guntapong, Ratigorn; Upachai, Sompong; Singchai, Phakapun; Ide, Tomihiko; Fukuda, Saori; Ruchusatsawast, Kriangsak; Sriwantana, Busarawan; Tatsumi, Masashi; Motomura, Kazushi; Takeda, Naokazu; Murata, Takayuki; Sangkitporn, Somchai; Taniguchi, Koki; Yoshikawa, Tetsushi.
：: Journal of Medical Virology, 巻 92, 番号 2, 01.02.2020, p. 174-186.研究成果: Article
TY - JOUR
T1 - High prevalence of equine-like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand
AU - Tacharoenmuang, Ratana
AU - Komoto, Satoshi
AU - Guntapong, Ratigorn
AU - Upachai, Sompong
AU - Singchai, Phakapun
AU - Ide, Tomihiko
AU - Fukuda, Saori
AU - Ruchusatsawast, Kriangsak
AU - Sriwantana, Busarawan
AU - Tatsumi, Masashi
AU - Motomura, Kazushi
AU - Takeda, Naokazu
AU - Murata, Takayuki
AU - Sangkitporn, Somchai
AU - Taniguchi, Koki
AU - Yoshikawa, Tetsushi
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Group A rotavirus (RVA) is a major cause of acute gastroenteritis in infants and young children worldwide. This study aims to clarify the distribution of G/P types and genetic characteristics of RVAs circulating in Thailand. Between January 2014 and September 2016, 1867 stool specimens were collected from children and adults with acute gastroenteritis in six provinces in Thailand. RVAs were detected in 514/1867 (27.5%) stool specimens. G1P[8] (44.7%) was the most predominant genotype, followed by G3P[8] (33.7%), G2P[4] (11.5%), G8P[8] (7.0%), and G9P[8] (1.3%). Unusual G3P[9] (0.8%), G3P[10] (0.4%), G4P[6] (0.4%), and G10P[14] (0.2%) were also detected at low frequencies. The predominant genotype, G1P[8] (64.4%), in 2014 decreased to 6.1% in 2016. In contrast, the frequency of G3P[8] markedly increased from 5.5% in 2014 to 65.3% in 2015 and 89.8% in 2016. On polyacrylamide gel electrophoresis, most (135/140; 96.4%) of the G3P[8] strains exhibited a short RNA profile. Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine-like G3P[8] strains. On phylogenetic analysis of genome segments of two representative Thai equine-like G3P[8] strains, it was noteworthy that they possessed distinct NSP4 genes, one bovine-like and the other human-like. Thus, we found that characteristic equine-like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand.
AB - Group A rotavirus (RVA) is a major cause of acute gastroenteritis in infants and young children worldwide. This study aims to clarify the distribution of G/P types and genetic characteristics of RVAs circulating in Thailand. Between January 2014 and September 2016, 1867 stool specimens were collected from children and adults with acute gastroenteritis in six provinces in Thailand. RVAs were detected in 514/1867 (27.5%) stool specimens. G1P[8] (44.7%) was the most predominant genotype, followed by G3P[8] (33.7%), G2P[4] (11.5%), G8P[8] (7.0%), and G9P[8] (1.3%). Unusual G3P[9] (0.8%), G3P[10] (0.4%), G4P[6] (0.4%), and G10P[14] (0.2%) were also detected at low frequencies. The predominant genotype, G1P[8] (64.4%), in 2014 decreased to 6.1% in 2016. In contrast, the frequency of G3P[8] markedly increased from 5.5% in 2014 to 65.3% in 2015 and 89.8% in 2016. On polyacrylamide gel electrophoresis, most (135/140; 96.4%) of the G3P[8] strains exhibited a short RNA profile. Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine-like G3P[8] strains. On phylogenetic analysis of genome segments of two representative Thai equine-like G3P[8] strains, it was noteworthy that they possessed distinct NSP4 genes, one bovine-like and the other human-like. Thus, we found that characteristic equine-like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand.
UR - http://www.scopus.com/inward/record.url?scp=85073971231&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85073971231&partnerID=8YFLogxK
U2 - 10.1002/jmv.25591
DO - 10.1002/jmv.25591
M3 - Article
C2 - 31498444
AN - SCOPUS:85073971231
VL - 92
SP - 174
EP - 186
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 2
ER -