Highly sensitive histamine-sensitization test for residual activity of pertussis toxin in acellular pertussis vaccine

Masaki Ochiai, Akihiko Yamamoto, Michiyo Kataoka, Hiromi Toyoizumi, Yoshichika Arakawa, Yoshinobu Horiuchi

研究成果: ジャーナルへの寄稿学術論文査読

22 被引用数 (Scopus)

抄録

Histamine-sensitization test method based on histamine-sensitizing death is widely used for controlling residual activity of pertussis toxin in acellular pertussis vaccines. The test method evaluates the residual activity according to the death of mice injected with a test vaccine after histamine challenge and the test result, therefore, depends on the sensitivity of mice. A highly sensitive test method based on change in rectal temperature of mice has been used in Japan for many years but has limited feasibility in other countries. We examined the possibility of a test method using dermal temperature measured by infrared thermometer to reduce animal suffering instead of rectal temperature. The dermal temperature method was shown to be as sensitive as the rectal temperature method. Furthermore, the dermal as well as rectal temperature methods can evaluate the activity of a test vaccine in relative to a reference preparation so as to allow direct comparison of the test results among different laboratories. The activity by means of the dermal temperature method was also found to be well consistent with that by the rectal temperature method.

本文言語英語
ページ(範囲)259-264
ページ数6
ジャーナルBiologicals
35
4
DOI
出版ステータス出版済み - 10-2007
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー
  • 免疫学および微生物学一般
  • 薬理学

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