Histone deacetylase inhibitor attenuates neurotoxicity of clioquinol in PC12 cells

Takao Fukui, Kunihiko Asakura, Chika Hikichi, Tomomasa Ishikawa, Rie Murai, Seiko Hirota, Ken ichiro Murate, Madoko Kizawa, Akihiro Ueda, Shinji Ito, Tatsuro Mutoh

研究成果: ジャーナルへの寄稿学術論文査読

13 被引用数 (Scopus)


Clioquinol is considered to be a causative agent of subacute myelo-optico neuropathy (SMON), although the pathogenesis of SMON is yet to be elucidated. We have previously shown that clioquinol inhibits nerve growth factor (NGF)-induced Trk autophosphorylation in PC12 cells transformed with human Trk cDNA. To explore the further mechanism of neuronal damage by clioquinol, we evaluated the acetylation status of histones in PC12 cells. Clioquinol reduced the level of histone acetylation, and the histone deacetylase (HDAC) inhibitor Trichostatin A upregulated acetylated histones and prevented the neuronal cell damage caused by clioquinol. In addition, treatment with HDAC inhibitor decreased neurite retraction and restored the inhibition of NGF-induced Trk autophosphorylation by clioquinol. Thus, clioquinol induced neuronal cell death via deacetylation of histones, and HDAC inhibitor alleviates the neurotoxicity of clioquinol. Clioquinol is now used as a potential medicine for malignancies and neurodegenerative diseases. Therefore, HDAC inhibitors can be used as a candidate medicine for the prevention of its side effects on neuronal cells.

出版ステータス出版済み - 04-05-2015

All Science Journal Classification (ASJC) codes

  • 毒物学


「Histone deacetylase inhibitor attenuates neurotoxicity of clioquinol in PC12 cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。