Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

Hiroaki Sugiyama; Kazuhisa Maeda; Masayuki Yamato; Ryuhei Hayashi; Takeshi Soma; Yasutaka Hayashida; Joseph Yang; Masayuki Shirakabe; Akifumi Matsuyama; Akihiko Kikuchi; Yoshiki Sawa; Teruo Okano; Yasuo Tano; Kohji Nishida

doi:10.1002/term.111

Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

Hiroaki Sugiyama
, Kazuhisa Maeda
, Masayuki Yamato
, Ryuhei Hayashi
, Takeshi Soma
, Yasutaka Hayashida
, Joseph Yang
, Masayuki Shirakabe
, Akifumi Matsuyama
, Akihiko Kikuchi
, Yoshiki Sawa
, Teruo Okano
, Yasuo Tano
, Kohji Nishida

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

28 被引用数 (Scopus)

抄録

We examined a novel human feeder cell layer of mesenchymal stem cells harvested from human adipose tissues. Gene expression analyses and colony-forming assay with human primary epithelial cells showed that the adipose tissue-derived mesenchymal stem cells produced various factors to support epithelial stem/progenitor maintenance and cell growth. Using the mesenchymal stem cells as novel feeder layers, transplantable epithelial cell sheets could be effectively generated ex vivo on temperature-responsive cell-culture surfaces.

本文言語	英語
ページ（範囲）	445-449
ページ数	5
ジャーナル	Journal of Tissue Engineering and Regenerative Medicine
巻	2
号	7
DOI	https://doi.org/10.1002/term.111
出版ステータス	出版済み - 10-2008

All Science Journal Classification (ASJC) codes

医学（その他）
生体材料
生体医工学

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10.1002/term.111

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Sugiyama, H., Maeda, K., Yamato, M., Hayashi, R., Soma, T., Hayashida, Y., Yang, J., Shirakabe, M., Matsuyama, A., Kikuchi, A., Sawa, Y., Okano, T., Tano, Y., & Nishida, K. (2008). Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells. Journal of Tissue Engineering and Regenerative Medicine, 2(7), 445-449. https://doi.org/10.1002/term.111

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abstract = "We examined a novel human feeder cell layer of mesenchymal stem cells harvested from human adipose tissues. Gene expression analyses and colony-forming assay with human primary epithelial cells showed that the adipose tissue-derived mesenchymal stem cells produced various factors to support epithelial stem/progenitor maintenance and cell growth. Using the mesenchymal stem cells as novel feeder layers, transplantable epithelial cell sheets could be effectively generated ex vivo on temperature-responsive cell-culture surfaces.",

author = "Hiroaki Sugiyama and Kazuhisa Maeda and Masayuki Yamato and Ryuhei Hayashi and Takeshi Soma and Yasutaka Hayashida and Joseph Yang and Masayuki Shirakabe and Akifumi Matsuyama and Akihiko Kikuchi and Yoshiki Sawa and Teruo Okano and Yasuo Tano and Kohji Nishida",

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Sugiyama, H, Maeda, K, Yamato, M, Hayashi, R, Soma, T, Hayashida, Y, Yang, J, Shirakabe, M, Matsuyama, A, Kikuchi, A, Sawa, Y, Okano, T, Tano, Y & Nishida, K 2008, 'Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells', Journal of Tissue Engineering and Regenerative Medicine, vol. 2, no. 7, pp. 445-449. https://doi.org/10.1002/term.111

TY - JOUR

T1 - Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

AU - Sugiyama, Hiroaki

AU - Maeda, Kazuhisa

AU - Yamato, Masayuki

AU - Hayashi, Ryuhei

AU - Soma, Takeshi

AU - Hayashida, Yasutaka

AU - Yang, Joseph

AU - Shirakabe, Masayuki

AU - Matsuyama, Akifumi

AU - Kikuchi, Akihiko

AU - Sawa, Yoshiki

AU - Okano, Teruo

AU - Tano, Yasuo

AU - Nishida, Kohji

PY - 2008/10

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AB - We examined a novel human feeder cell layer of mesenchymal stem cells harvested from human adipose tissues. Gene expression analyses and colony-forming assay with human primary epithelial cells showed that the adipose tissue-derived mesenchymal stem cells produced various factors to support epithelial stem/progenitor maintenance and cell growth. Using the mesenchymal stem cells as novel feeder layers, transplantable epithelial cell sheets could be effectively generated ex vivo on temperature-responsive cell-culture surfaces.

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JO - Journal of Tissue Engineering and Regenerative Medicine

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Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

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All Science Journal Classification (ASJC) codes

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