Human T cells recognize peptides in the context of MHC class I and II molecules. It is not completely clear how the disparity in MHC molecules between species affects the T cell MHC interaction. We have generated swine MHC restricted human cytolytic T lymphocytes (CTL) which can directly recognize and differentiate swine leukocyte antigens (SLA) expressed on yy and zz haplotypes of Yucatan breed of miniature swine. The CTL killing is SLA class I restricted and human CD8 is directly involved in the interaction with SLA molecules. Peptides eluted from affinity purified SLA class I molecules from the zz haplotype, but not from the yy haplotype, increase the killing of 721.221 and 721.220 cell lines expressing a SLA class I heavy chain (SLA.1Z) from the zz haplotype. In addition, porcine aortic endothelial cells and SLA.1Z expressed 721.221 and 721.220 cells are not recognized by the T cells if the peptides are stripped from the SLA by acid treatment. Adding back the SLA bound porcine peptides restores the susceptibility to killing in the target cells. In conclusion, our results suggest that despite the difference in MHC sequences between species, recognition of xenogeneic MHC from miniature swine by human T cells is similar to direct allo-recognition. In addition, such TCR-xenogeneic MHC interaction is influenced by the xenogeneic peptides bound to the peptide binding groove of MHC.
|出版ステータス||Published - 20-03-1998|
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