Investigations on the general characteristics of human astrocytoma cell line NAC-1 revealed neuroblastoma growth inhibitory activity in conditioned medium. Neuroblastoma growth inhibitory factor (NGIF) was partially purified by Econo Q, Econo CM, and Superose 12 column chromatography. The protein is weakly basic with an estimated Mr of 120,000, possibly having an Mr 60,000 dimeric structure. NGIF inhibits the growth of neurobalstoma cell lines but has no effect on morphology nor does it produce any change in the growth of human glioblastoma cell lines. Interstingly, NGIF appears to promote survival and neurite outgrowth of embryonal rat cortical neurons. These neurotrophic properties suggest a role for NGIF in the development of the nervous system.
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