TY - JOUR
T1 - Iba1-expressing microglia respond to herpes simplex virus infection in the mouse trigeminal ganglion
AU - Mori, Isamu
AU - Goshima, Fumi
AU - Koshizuka, Tetsuo
AU - Imai, Yoshinori
AU - Kohsaka, Shinichi
AU - Koide, Naoki
AU - Sugiyama, Tsuyoshi
AU - Yoshida, Tomoaki
AU - Yokochi, Takashi
AU - Kimura, Yoshinobu
AU - Nishiyama, Yukihiro
N1 - Funding Information:
We would like to thank E. Iwata and T. Tsuruguchi for technical assistance. I. Mori was the recipient of an Encouragement of Young Scientist Award from the Nakanihon Infectious Diseases Research Foundation, Japan.
PY - 2003/12/12
Y1 - 2003/12/12
N2 - Microglial response in the trigeminal ganglion of mice corneally inoculated with herpes simplex virus (HSV) was investigated. Virus-infected neurons of the trigeminal ganglion did not exhibit apoptotic signal, while those of the trigeminal sensory brainstem nucleus did. Cells expressing ionized calcium binding adapter molecule 1 (Iba1), a specific marker of microglia/macrophages, increased in number in the virally infected region of the trigeminal ganglion, with morphological transformation to an activated phenotype, frequently detected as perineural satellites. Further microglial transformation to macropahges was not evident. Iba1-immunopositive perineural satellites also appeared in the vicinity of virally infected region. Such activated microglia expressed basic fibroblast growth factor (bFGF) molecules. The reverse transcription-polymerase chain reaction (RT-PCR) detected upregulated synthesis of mRNA for bFGF in the trigeminal ganglion. In contrast, in the trigeminal sensory brainstem nucleus, a small number of bFGF-producing cells appeared only in the vicinity of virally infected area. Collectively, Iba1-bearing microglia exist in the mouse trigeminal ganglion and respond to herpes simplex virus infection, most likely conferring neuroprotective functions upon the trigeminal ganglion.
AB - Microglial response in the trigeminal ganglion of mice corneally inoculated with herpes simplex virus (HSV) was investigated. Virus-infected neurons of the trigeminal ganglion did not exhibit apoptotic signal, while those of the trigeminal sensory brainstem nucleus did. Cells expressing ionized calcium binding adapter molecule 1 (Iba1), a specific marker of microglia/macrophages, increased in number in the virally infected region of the trigeminal ganglion, with morphological transformation to an activated phenotype, frequently detected as perineural satellites. Further microglial transformation to macropahges was not evident. Iba1-immunopositive perineural satellites also appeared in the vicinity of virally infected region. Such activated microglia expressed basic fibroblast growth factor (bFGF) molecules. The reverse transcription-polymerase chain reaction (RT-PCR) detected upregulated synthesis of mRNA for bFGF in the trigeminal ganglion. In contrast, in the trigeminal sensory brainstem nucleus, a small number of bFGF-producing cells appeared only in the vicinity of virally infected area. Collectively, Iba1-bearing microglia exist in the mouse trigeminal ganglion and respond to herpes simplex virus infection, most likely conferring neuroprotective functions upon the trigeminal ganglion.
KW - Basic fibroblast growth factor
KW - Herpes simplex virus
KW - Iba1
KW - Microglia
KW - Trigeminal ganglion
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U2 - 10.1016/j.molbrainres.2003.10.003
DO - 10.1016/j.molbrainres.2003.10.003
M3 - Article
C2 - 14667577
AN - SCOPUS:10744228915
SN - 0169-328X
VL - 120
SP - 52
EP - 56
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -