Identification and validation of evolutionarily conserved unusually short pre-mRNA introns in the human genome

Makoto K. Shimada, Noriko Sasaki-Haraguchi, Akila Mayeda

研究成果: Article

6 引用 (Scopus)

抄録

According to the length distribution of human introns, there is a large population of short introns with a threshold of 65 nucleotides (nt) and a peak at 85 nt. Using human genome and transcriptome databases, we investigated the introns shorter than 66 nt, termed ultra-short introns, the identities of which are scarcely known. Here, we provide for the first time a list of bona fide human ultra-short introns, which have never been characterized elsewhere. By conducting BLAST searches of the databases, we screened 22 introns (37–65 nt) with conserved lengths and sequences among closely related species. We then provide experimental and bioinformatic evidence for the splicing of 15 introns, of which 12 introns were remarkably G-rich and 9 introns contained completely inefficient splice sites and/or branch sites. These unorthodox characteristics of ultra-short introns suggest that there are unknown splicing mechanisms that differ from the well-established mechanism.

元の言語English
ページ(範囲)10376-10388
ページ数13
ジャーナルInternational journal of molecular sciences
16
発行部数5
DOI
出版物ステータスPublished - 07-05-2015

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genome
RNA Precursors
nucleotides
Human Genome
Nucleotides
Introns
Genes
splicing
Bioinformatics
lists
conduction
thresholds
Messenger RNA
Databases
Conserved Sequence
Computational Biology
Transcriptome

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

これを引用

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