Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles

Ryu Miyagawa, Keiko Tano, R. I.E. Mizuno, Y. O. Nakamura, Kenichi Ijiri, Randeep Rakwal, Junko Shibato, Yoshinori Masuo, Akila Mayeda, Tetsuro Hirose, Nobuyoshi Akimitsu

研究成果: Article査読

152 被引用数 (Scopus)

抄録

MALAT-1 noncoding RNA is localized to nuclear speckles despite its mRNA-like characteristics. Here, we report the identification of several key factors that promote the localization of MALAT-1 to nuclear speckles and also provide evidence that MALAT-1 is involved in the regulation of gene expression. Heterokaryon assays revealed that MALAT-1 does not shuttle between the nucleus and cytoplasm. RNAi-mediated repression of the nuclear speckle proteins, RNPS1, SRm160, or IBP160, which are well-known mRNA processing factors, resulted in the diffusion of MALAT-1 to the nucleoplasm. We demonstrated that MALAT-1 contains two distinct elements directing transcripts to nuclear speckles, which were also capable of binding to RNPS1 in vitro. Depletion of MALAT-1 represses the expression of several genes. Taken together, our results suggest that RNPS1, SRm160, and IBP160 contribute to the localization of MALAT-1 to nuclear speckles, where MALAT-1 could be involved in regulating gene expression.

本文言語English
ページ(範囲)738-751
ページ数14
ジャーナルRNA
18
4
DOI
出版ステータスPublished - 04-2012

All Science Journal Classification (ASJC) codes

  • 分子生物学

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