Identification of functional polymorphisms in the promoter region of the human PICK1 gene and their association with methamphetamine psychosis

Daisuke Matsuzawa, Kenji Hashimoto, Ryosuke Miyatake, Yukihiko Shirayama, Eiji Shimizu, Kazuhisa Maeda, Yoichi Suzuki, Yoichi Mashimo, Yoshimoto Sekine, Toshiya Inada, Norio Ozaki, Nakao Iwata, Mutsuo Harano, Tokutaro Komiyama, Mitsuhiko Yamada, Ichiro Sora, Hiroshi Ujike, Akira Hata, Akira Sawa, Masaomi Iyo

研究成果: Article

29 引用 (Scopus)

抄録

Objective: Protein interacting with C-kinase-1 (PICK1) plays a role in the targeting and clustering of dopamine transporter, which is the primary target site for the abused drug methamphetamine. Based on the interaction of PICK1 with dopamine transporter, it is of particular interest to investigate the association between the PICK1 gene and methamphetamine abusers. Method: The authors studied the association between PICK1 gene polymorphisms and methamphetamine abusers in a Japanese group. Two hundred and eight methamphetamine abusers and 218 healthy comparison subjects were enrolled in the study. Furthermore, the authors also examined the effects of single nucleotide polymorphisms (SNPs) in the promoter and 5′-untranslated region on transcription levels of PICK1. Results: The authors identified four highly frequent SNPs, rs737622 (-332 C/G) and rs3026682 (-205 G/A) in the promoter region and rs713729 (T/A) in intron3 and rs2076369 (T/G) in intron4. Of these SNPs, rs713729 was significantly associated with methamphetamine abusers in general, and rs713729 and rs2076369 were significantly associated with those with spontaneous relapse of psychosis. Furthermore, haplotype analysis revealed that specific haplotypes of these SNPs were associated with methamphetamine abusers. A gene reporter assay revealed that the two SNPs in the promoter region significantly altered transcriptional activity. Conclusions: Our findings suggest that the PICK1 gene may be implicated in the susceptibility to spontaneous relapse of methamphetamine psychosis and that, as an intracellular adapter protein, PICK1 may play a role in the pathophysiology of methamphetamine psychosis.

元の言語English
ページ(範囲)1105-1114
ページ数10
ジャーナルAmerican Journal of Psychiatry
164
発行部数7
DOI
出版物ステータスPublished - 01-01-2007

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Methamphetamine
Genetic Promoter Regions
Psychotic Disorders
Phosphotransferases
Single Nucleotide Polymorphism
Genes
Proteins
Dopamine Plasma Membrane Transport Proteins
Haplotypes
Recurrence
Human PICk1 protein
5' Untranslated Regions
Reporter Genes
Cluster Analysis
Healthy Volunteers
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Psychiatry and Mental health

これを引用

Matsuzawa, Daisuke ; Hashimoto, Kenji ; Miyatake, Ryosuke ; Shirayama, Yukihiko ; Shimizu, Eiji ; Maeda, Kazuhisa ; Suzuki, Yoichi ; Mashimo, Yoichi ; Sekine, Yoshimoto ; Inada, Toshiya ; Ozaki, Norio ; Iwata, Nakao ; Harano, Mutsuo ; Komiyama, Tokutaro ; Yamada, Mitsuhiko ; Sora, Ichiro ; Ujike, Hiroshi ; Hata, Akira ; Sawa, Akira ; Iyo, Masaomi. / Identification of functional polymorphisms in the promoter region of the human PICK1 gene and their association with methamphetamine psychosis. :: American Journal of Psychiatry. 2007 ; 巻 164, 番号 7. pp. 1105-1114.
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title = "Identification of functional polymorphisms in the promoter region of the human PICK1 gene and their association with methamphetamine psychosis",
abstract = "Objective: Protein interacting with C-kinase-1 (PICK1) plays a role in the targeting and clustering of dopamine transporter, which is the primary target site for the abused drug methamphetamine. Based on the interaction of PICK1 with dopamine transporter, it is of particular interest to investigate the association between the PICK1 gene and methamphetamine abusers. Method: The authors studied the association between PICK1 gene polymorphisms and methamphetamine abusers in a Japanese group. Two hundred and eight methamphetamine abusers and 218 healthy comparison subjects were enrolled in the study. Furthermore, the authors also examined the effects of single nucleotide polymorphisms (SNPs) in the promoter and 5′-untranslated region on transcription levels of PICK1. Results: The authors identified four highly frequent SNPs, rs737622 (-332 C/G) and rs3026682 (-205 G/A) in the promoter region and rs713729 (T/A) in intron3 and rs2076369 (T/G) in intron4. Of these SNPs, rs713729 was significantly associated with methamphetamine abusers in general, and rs713729 and rs2076369 were significantly associated with those with spontaneous relapse of psychosis. Furthermore, haplotype analysis revealed that specific haplotypes of these SNPs were associated with methamphetamine abusers. A gene reporter assay revealed that the two SNPs in the promoter region significantly altered transcriptional activity. Conclusions: Our findings suggest that the PICK1 gene may be implicated in the susceptibility to spontaneous relapse of methamphetamine psychosis and that, as an intracellular adapter protein, PICK1 may play a role in the pathophysiology of methamphetamine psychosis.",
author = "Daisuke Matsuzawa and Kenji Hashimoto and Ryosuke Miyatake and Yukihiko Shirayama and Eiji Shimizu and Kazuhisa Maeda and Yoichi Suzuki and Yoichi Mashimo and Yoshimoto Sekine and Toshiya Inada and Norio Ozaki and Nakao Iwata and Mutsuo Harano and Tokutaro Komiyama and Mitsuhiko Yamada and Ichiro Sora and Hiroshi Ujike and Akira Hata and Akira Sawa and Masaomi Iyo",
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doi = "10.1176/ajp.2007.164.7.1105",
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Matsuzawa, D, Hashimoto, K, Miyatake, R, Shirayama, Y, Shimizu, E, Maeda, K, Suzuki, Y, Mashimo, Y, Sekine, Y, Inada, T, Ozaki, N, Iwata, N, Harano, M, Komiyama, T, Yamada, M, Sora, I, Ujike, H, Hata, A, Sawa, A & Iyo, M 2007, 'Identification of functional polymorphisms in the promoter region of the human PICK1 gene and their association with methamphetamine psychosis', American Journal of Psychiatry, 巻. 164, 番号 7, pp. 1105-1114. https://doi.org/10.1176/ajp.2007.164.7.1105

Identification of functional polymorphisms in the promoter region of the human PICK1 gene and their association with methamphetamine psychosis. / Matsuzawa, Daisuke; Hashimoto, Kenji; Miyatake, Ryosuke; Shirayama, Yukihiko; Shimizu, Eiji; Maeda, Kazuhisa; Suzuki, Yoichi; Mashimo, Yoichi; Sekine, Yoshimoto; Inada, Toshiya; Ozaki, Norio; Iwata, Nakao; Harano, Mutsuo; Komiyama, Tokutaro; Yamada, Mitsuhiko; Sora, Ichiro; Ujike, Hiroshi; Hata, Akira; Sawa, Akira; Iyo, Masaomi.

:: American Journal of Psychiatry, 巻 164, 番号 7, 01.01.2007, p. 1105-1114.

研究成果: Article

TY - JOUR

T1 - Identification of functional polymorphisms in the promoter region of the human PICK1 gene and their association with methamphetamine psychosis

AU - Matsuzawa, Daisuke

AU - Hashimoto, Kenji

AU - Miyatake, Ryosuke

AU - Shirayama, Yukihiko

AU - Shimizu, Eiji

AU - Maeda, Kazuhisa

AU - Suzuki, Yoichi

AU - Mashimo, Yoichi

AU - Sekine, Yoshimoto

AU - Inada, Toshiya

AU - Ozaki, Norio

AU - Iwata, Nakao

AU - Harano, Mutsuo

AU - Komiyama, Tokutaro

AU - Yamada, Mitsuhiko

AU - Sora, Ichiro

AU - Ujike, Hiroshi

AU - Hata, Akira

AU - Sawa, Akira

AU - Iyo, Masaomi

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Objective: Protein interacting with C-kinase-1 (PICK1) plays a role in the targeting and clustering of dopamine transporter, which is the primary target site for the abused drug methamphetamine. Based on the interaction of PICK1 with dopamine transporter, it is of particular interest to investigate the association between the PICK1 gene and methamphetamine abusers. Method: The authors studied the association between PICK1 gene polymorphisms and methamphetamine abusers in a Japanese group. Two hundred and eight methamphetamine abusers and 218 healthy comparison subjects were enrolled in the study. Furthermore, the authors also examined the effects of single nucleotide polymorphisms (SNPs) in the promoter and 5′-untranslated region on transcription levels of PICK1. Results: The authors identified four highly frequent SNPs, rs737622 (-332 C/G) and rs3026682 (-205 G/A) in the promoter region and rs713729 (T/A) in intron3 and rs2076369 (T/G) in intron4. Of these SNPs, rs713729 was significantly associated with methamphetamine abusers in general, and rs713729 and rs2076369 were significantly associated with those with spontaneous relapse of psychosis. Furthermore, haplotype analysis revealed that specific haplotypes of these SNPs were associated with methamphetamine abusers. A gene reporter assay revealed that the two SNPs in the promoter region significantly altered transcriptional activity. Conclusions: Our findings suggest that the PICK1 gene may be implicated in the susceptibility to spontaneous relapse of methamphetamine psychosis and that, as an intracellular adapter protein, PICK1 may play a role in the pathophysiology of methamphetamine psychosis.

AB - Objective: Protein interacting with C-kinase-1 (PICK1) plays a role in the targeting and clustering of dopamine transporter, which is the primary target site for the abused drug methamphetamine. Based on the interaction of PICK1 with dopamine transporter, it is of particular interest to investigate the association between the PICK1 gene and methamphetamine abusers. Method: The authors studied the association between PICK1 gene polymorphisms and methamphetamine abusers in a Japanese group. Two hundred and eight methamphetamine abusers and 218 healthy comparison subjects were enrolled in the study. Furthermore, the authors also examined the effects of single nucleotide polymorphisms (SNPs) in the promoter and 5′-untranslated region on transcription levels of PICK1. Results: The authors identified four highly frequent SNPs, rs737622 (-332 C/G) and rs3026682 (-205 G/A) in the promoter region and rs713729 (T/A) in intron3 and rs2076369 (T/G) in intron4. Of these SNPs, rs713729 was significantly associated with methamphetamine abusers in general, and rs713729 and rs2076369 were significantly associated with those with spontaneous relapse of psychosis. Furthermore, haplotype analysis revealed that specific haplotypes of these SNPs were associated with methamphetamine abusers. A gene reporter assay revealed that the two SNPs in the promoter region significantly altered transcriptional activity. Conclusions: Our findings suggest that the PICK1 gene may be implicated in the susceptibility to spontaneous relapse of methamphetamine psychosis and that, as an intracellular adapter protein, PICK1 may play a role in the pathophysiology of methamphetamine psychosis.

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U2 - 10.1176/ajp.2007.164.7.1105

DO - 10.1176/ajp.2007.164.7.1105

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VL - 164

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EP - 1114

JO - American Journal of Psychiatry

JF - American Journal of Psychiatry

SN - 0002-953X

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