Identification of live hair cells in rat cochlear sections in culture with FM1-43 fluorescent dye

Jun Fukuda, Hisako Ishimine, Motohide Tokunaga

研究成果: ジャーナルへの寄稿学術論文査読

7 被引用数 (Scopus)


Cochlear hair cells are presumed to live in culture for many days, yet they are difficult to identify in cultured tissues. We stained hair cells in cochlear sections with FM1-43 and cultured them in collagen matrix. Three rows of outer hair cells and a single row of inner ones were distinguished by staining with FM1-43. Fixation of the sections with paraformaldehyde caused loss of the FM1-43 fluorescence, indicating that FM1-43 stained only live hair cells. In sections cultured for 48 h, almost all hair cells were still positive with FM1-43. Culture with gentamycin caused loss of FM1-43-positive cells. In serum-free, long-term cultures (15 days) performed without antibiotics or neurotrophins, the row alignment of FM1-43-positive hair cells was still maintained. Membranous labyrinth-like vacuoles enveloping hair cells were formed in the collagen matrix. Accordingly, FM1-43 is an efficient marker for identifying live hair cells in cultured tissues. Moreover, cochlear hair cells are revealed to live for weeks in serum-free culture without exogenous neurotrophins.

ジャーナルNeuroscience Letters
出版ステータス出版済み - 23-01-2004

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)


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