Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia

Satoshi Kawai, Takuma Fujii, Iwao Kukimoto, Hiroya Yamada, Naoki Yamamoto, Makoto Kuroda, Sayaka Otani, Ryoko Ichikawa, Eiji Nishio, Yutaka Torii, Aya Iwata

研究成果: Article

6 引用 (Scopus)

抄録

microRNAs (miRNAs) play important roles in regulation of gene expression during cervical carcinogenesis. We investigated expression profiles of miRNAs in cervical cancer and its precursor lesions by utilizing cervical mucus. Cervical mucus was collected from 230 patients with a normal cervix, cervical intraepithelial neoplasia (CIN), squamous cell carcinoma (SCC), or adenocarcinoma (AD). The levels of miRNA in the mucus were quantified by miRNA array and real-time reverse-transcriptase polymerase chain reaction (RT-PCR). The performance for detecting diseases was statistically analysed. The expression of miRNAs was further validated in the surgical tissues of enrolled patients. Four miRNAs (miR-126-3p, -20b-5p, -451a, and -144-3p) were significantly up-regulated in SCC and AD compared with normal, and their expression levels correlated with disease severity and high-risk human papillomavirus infection. Receiver operating characteristic curve analyses revealed that the area under the curve values for miR-126-3p, -20b-5p, -451a, and -144-3p were 0.89, 0.90, 0.94, and 0.93, respectively, for SCC plus AD compared with normal, showing high accuracy of cancer detection. Real-time RT-PCR analyses confirmed the expression of these four miRNAs in frozen tissues from cervical cancer. miR-126-3p, -20b-5p, -451a, and -144-3p in cervical mucus are promising biomarkers for cervical cancer and high-grade CINs.

元の言語English
記事番号7070
ジャーナルScientific reports
8
発行部数1
DOI
出版物ステータスPublished - 01-12-2018

Fingerprint

Cervix Mucus
MicroRNAs
Neoplasms
Uterine Cervical Neoplasms
Squamous Cell Carcinoma
Adenocarcinoma
Reverse Transcriptase Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Cervical Intraepithelial Neoplasia
Papillomavirus Infections
Gene Expression Regulation
Mucus
Cervix Uteri
ROC Curve
Area Under Curve
Carcinogenesis
Biomarkers

All Science Journal Classification (ASJC) codes

  • General

これを引用

Kawai, Satoshi ; Fujii, Takuma ; Kukimoto, Iwao ; Yamada, Hiroya ; Yamamoto, Naoki ; Kuroda, Makoto ; Otani, Sayaka ; Ichikawa, Ryoko ; Nishio, Eiji ; Torii, Yutaka ; Iwata, Aya. / Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia. :: Scientific reports. 2018 ; 巻 8, 番号 1.
@article{0545a4e9349048fa9ae5e7d3c2f27d36,
title = "Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia",
abstract = "microRNAs (miRNAs) play important roles in regulation of gene expression during cervical carcinogenesis. We investigated expression profiles of miRNAs in cervical cancer and its precursor lesions by utilizing cervical mucus. Cervical mucus was collected from 230 patients with a normal cervix, cervical intraepithelial neoplasia (CIN), squamous cell carcinoma (SCC), or adenocarcinoma (AD). The levels of miRNA in the mucus were quantified by miRNA array and real-time reverse-transcriptase polymerase chain reaction (RT-PCR). The performance for detecting diseases was statistically analysed. The expression of miRNAs was further validated in the surgical tissues of enrolled patients. Four miRNAs (miR-126-3p, -20b-5p, -451a, and -144-3p) were significantly up-regulated in SCC and AD compared with normal, and their expression levels correlated with disease severity and high-risk human papillomavirus infection. Receiver operating characteristic curve analyses revealed that the area under the curve values for miR-126-3p, -20b-5p, -451a, and -144-3p were 0.89, 0.90, 0.94, and 0.93, respectively, for SCC plus AD compared with normal, showing high accuracy of cancer detection. Real-time RT-PCR analyses confirmed the expression of these four miRNAs in frozen tissues from cervical cancer. miR-126-3p, -20b-5p, -451a, and -144-3p in cervical mucus are promising biomarkers for cervical cancer and high-grade CINs.",
author = "Satoshi Kawai and Takuma Fujii and Iwao Kukimoto and Hiroya Yamada and Naoki Yamamoto and Makoto Kuroda and Sayaka Otani and Ryoko Ichikawa and Eiji Nishio and Yutaka Torii and Aya Iwata",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-018-25310-1",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia. / Kawai, Satoshi; Fujii, Takuma; Kukimoto, Iwao; Yamada, Hiroya; Yamamoto, Naoki; Kuroda, Makoto; Otani, Sayaka; Ichikawa, Ryoko; Nishio, Eiji; Torii, Yutaka; Iwata, Aya.

:: Scientific reports, 巻 8, 番号 1, 7070, 01.12.2018.

研究成果: Article

TY - JOUR

T1 - Identification of miRNAs in cervical mucus as a novel diagnostic marker for cervical neoplasia

AU - Kawai, Satoshi

AU - Fujii, Takuma

AU - Kukimoto, Iwao

AU - Yamada, Hiroya

AU - Yamamoto, Naoki

AU - Kuroda, Makoto

AU - Otani, Sayaka

AU - Ichikawa, Ryoko

AU - Nishio, Eiji

AU - Torii, Yutaka

AU - Iwata, Aya

PY - 2018/12/1

Y1 - 2018/12/1

N2 - microRNAs (miRNAs) play important roles in regulation of gene expression during cervical carcinogenesis. We investigated expression profiles of miRNAs in cervical cancer and its precursor lesions by utilizing cervical mucus. Cervical mucus was collected from 230 patients with a normal cervix, cervical intraepithelial neoplasia (CIN), squamous cell carcinoma (SCC), or adenocarcinoma (AD). The levels of miRNA in the mucus were quantified by miRNA array and real-time reverse-transcriptase polymerase chain reaction (RT-PCR). The performance for detecting diseases was statistically analysed. The expression of miRNAs was further validated in the surgical tissues of enrolled patients. Four miRNAs (miR-126-3p, -20b-5p, -451a, and -144-3p) were significantly up-regulated in SCC and AD compared with normal, and their expression levels correlated with disease severity and high-risk human papillomavirus infection. Receiver operating characteristic curve analyses revealed that the area under the curve values for miR-126-3p, -20b-5p, -451a, and -144-3p were 0.89, 0.90, 0.94, and 0.93, respectively, for SCC plus AD compared with normal, showing high accuracy of cancer detection. Real-time RT-PCR analyses confirmed the expression of these four miRNAs in frozen tissues from cervical cancer. miR-126-3p, -20b-5p, -451a, and -144-3p in cervical mucus are promising biomarkers for cervical cancer and high-grade CINs.

AB - microRNAs (miRNAs) play important roles in regulation of gene expression during cervical carcinogenesis. We investigated expression profiles of miRNAs in cervical cancer and its precursor lesions by utilizing cervical mucus. Cervical mucus was collected from 230 patients with a normal cervix, cervical intraepithelial neoplasia (CIN), squamous cell carcinoma (SCC), or adenocarcinoma (AD). The levels of miRNA in the mucus were quantified by miRNA array and real-time reverse-transcriptase polymerase chain reaction (RT-PCR). The performance for detecting diseases was statistically analysed. The expression of miRNAs was further validated in the surgical tissues of enrolled patients. Four miRNAs (miR-126-3p, -20b-5p, -451a, and -144-3p) were significantly up-regulated in SCC and AD compared with normal, and their expression levels correlated with disease severity and high-risk human papillomavirus infection. Receiver operating characteristic curve analyses revealed that the area under the curve values for miR-126-3p, -20b-5p, -451a, and -144-3p were 0.89, 0.90, 0.94, and 0.93, respectively, for SCC plus AD compared with normal, showing high accuracy of cancer detection. Real-time RT-PCR analyses confirmed the expression of these four miRNAs in frozen tissues from cervical cancer. miR-126-3p, -20b-5p, -451a, and -144-3p in cervical mucus are promising biomarkers for cervical cancer and high-grade CINs.

UR - http://www.scopus.com/inward/record.url?scp=85046618939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046618939&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-25310-1

DO - 10.1038/s41598-018-25310-1

M3 - Article

C2 - 29728572

AN - SCOPUS:85046618939

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 7070

ER -