Identification of multispecific organic anion transporter 2 expressed predominantly in the liver

Takashi Sekine; Seok Ho Cha; Minoru Tsuda; Nopporn Apiwattanakul; Noriko Nakajima; Yoshikatsu Kanai; Hitoshi Endou

doi:10.1016/S0014-5793(98)00585-7

Identification of multispecific organic anion transporter 2 expressed predominantly in the liver

Takashi Sekine
, Seok Ho Cha
, Minoru Tsuda
, Nopporn Apiwattanakul
, Noriko Nakajima
, Yoshikatsu Kanai
, Hitoshi Endou

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

320 被引用数 (Scopus)

抄録

In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42% identity to that of the renal multispecific organic anion transporter, OAT1. When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE₂, dicarboxylates and p-aminohippurate. [¹⁴C]Salicylate uptake via NLT was saturable (K(m) = 88.8 ± 23.4 μM) and sodium-independent. Expression of the mRNA of NLT was detected in the liver and kidney (liver >> kidney). We propose that NLT be renamed OAT2.

本文言語	英語
ページ（範囲）	179-182
ページ数	4
ジャーナル	FEBS Letters
巻	429
号	2
DOI	https://doi.org/10.1016/S0014-5793(98)00585-7
出版ステータス	出版済み - 12-06-1998
外部発表	はい

All Science Journal Classification (ASJC) codes

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/S0014-5793(98)00585-7

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title = "Identification of multispecific organic anion transporter 2 expressed predominantly in the liver",

abstract = "In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42\% identity to that of the renal multispecific organic anion transporter, OAT1. When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE2, dicarboxylates and p-aminohippurate. [14C]Salicylate uptake via NLT was saturable (K(m) = 88.8 ± 23.4 μM) and sodium-independent. Expression of the mRNA of NLT was detected in the liver and kidney (liver >> kidney). We propose that NLT be renamed OAT2.",

keywords = "Liver, Multispecific transporter, Organic anion, Pharmacokinetics",

author = "Takashi Sekine and Cha, \{Seok Ho\} and Minoru Tsuda and Nopporn Apiwattanakul and Noriko Nakajima and Yoshikatsu Kanai and Hitoshi Endou",

year = "1998",

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T1 - Identification of multispecific organic anion transporter 2 expressed predominantly in the liver

AU - Sekine, Takashi

AU - Cha, Seok Ho

AU - Tsuda, Minoru

AU - Apiwattanakul, Nopporn

AU - Nakajima, Noriko

AU - Kanai, Yoshikatsu

AU - Endou, Hitoshi

PY - 1998/6/12

Y1 - 1998/6/12

N2 - In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42% identity to that of the renal multispecific organic anion transporter, OAT1. When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE2, dicarboxylates and p-aminohippurate. [14C]Salicylate uptake via NLT was saturable (K(m) = 88.8 ± 23.4 μM) and sodium-independent. Expression of the mRNA of NLT was detected in the liver and kidney (liver >> kidney). We propose that NLT be renamed OAT2.

AB - In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42% identity to that of the renal multispecific organic anion transporter, OAT1. When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE2, dicarboxylates and p-aminohippurate. [14C]Salicylate uptake via NLT was saturable (K(m) = 88.8 ± 23.4 μM) and sodium-independent. Expression of the mRNA of NLT was detected in the liver and kidney (liver >> kidney). We propose that NLT be renamed OAT2.

KW - Liver

KW - Multispecific transporter

KW - Organic anion

KW - Pharmacokinetics

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UR - https://www.scopus.com/pages/publications/17144449057#tab=citedBy

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DO - 10.1016/S0014-5793(98)00585-7

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SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

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Identification of multispecific organic anion transporter 2 expressed predominantly in the liver

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All Science Journal Classification (ASJC) codes

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