Identification of novel first exons in Ad4BP/SF-1 (NR5A1) gene and their tissue- and species-specific usage

Rina Kimura, Hironori Yoshii, Masatoshi Nomura, Naoe Kotomura, Tokuo Mukai, Satoru Ishihara, Koichi Ohba, Toshihiko Yanase, Osamu Gotoh, Hajime Nawata, Ken ichirou Morohashi

研究成果: ジャーナルへの寄稿学術論文査読

21 被引用数 (Scopus)


It has been demonstrated that the mammalian Ad4BP/SF-1 (NR5A1) gene is regulated precisely in sex, tissue, and developmental stage specific manners. To clarify the complex transcriptional regulation, we investigated in the present study whether the gene transcription is regulated by multiple promoters accompanied by noncoding first exons. Novel first exons (Io and Ig) were identified downstream of the already identified exon Ia. Nucleotide sequences revealed that Ia and Ig exons were well conserved, whereas Io exon was less conserved among the mouse, rat, and human genes. Interestingly, the splice donor of the mouse and human Io and human Ig exons do not satisfy the consensus sequence. Transcripts containing Ia, Io, and Ig were detected in all rat tissues examined, while the transcript containing Io was undetectable in the corresponding tissues of mice. The lack of exon Io usage in the mouse was confirmed by transient transfection assays with cultured cells. Quantitative RT-PCR analysis revealed that the transcript containing Ig exon was the main product in the pituitary but significantly less in the spleen, suggesting that the regulation of Ad4BP/SF-1 gene transcription in the pituitary and spleen is distinct from that of other tissues. The above findings, together with the structural abnormality at the splice donor site, suggest that acquisition of the multiple first exons enables the Ad4BP/SF-1 gene to be regulated differentially in different animal species and in different tissues in the same animal. (C) 2000 Academic Press.

ジャーナルBiochemical and Biophysical Research Communications
出版ステータス出版済み - 11-11-2000

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学


「Identification of novel first exons in Ad4BP/SF-1 (NR5A1) gene and their tissue- and species-specific usage」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。