@article{9393e854f7af4e7b9ed58b5f8a783f25,
title = "Identification of proteasomal catalytic subunit PSMA6 as a therapeutic target for lung cancer",
abstract = "To identify potential therapeutic targets for lung cancer, we performed semi-genome-wide shRNA screening combined with the utilization of genome-wide expression and copy number data. shRNA screening targeting 5043 genes in NCI-H460 identified 51 genes as candidates. Pathway analysis revealed that the 51 genes were enriched for the five pathways, including ribosome, proteasome, RNA polymerase, pyrimidine metabolism and spliceosome pathways. We focused on the proteasome pathway that involved six candidate genes because its activation has been demonstrated in diverse human malignancies, including lung cancer. Microarray expression and array CGH data showed that PSMA6, a proteasomal subunit of a 20S catalytic core complex, was highly expressed in lung cancer cell lines, with recurrent gene amplifications in some cases. Therefore, we further examined the roles of PSMA6 in lung cancer. Silencing of PSMA6 induced apoptosis or G2/M cell cycle arrest in cancer cell lines but not in an immortalized normal lung cell line. These results suggested that PSMA6 serves as an attractive target with a high therapeutic index for lung cancer.",
author = "Tomohiko Kakumu and Mitsuo Sato and Daiki Goto and Toshio Kato and Naoyuki Yogo and Tetsunari Hase and Masahiro Morise and Takayuki Fukui and Kohei Yokoi and Yoshitaka Sekido and Luc Girard and Minna, {John D.} and Byers, {Lauren A.} and Heymach, {John V.} and Coombes, {Kevin R.} and Masashi Kondo and Yoshinori Hasegawa",
note = "Funding Information: Funding Information Grant-in-Aid for Exploratory Research (Grant/Award Number: 26670416), Grant-in-Aid for Scientific Research (C) (Grant/Award Number: 23591144), Grant-in-Aid for Scientific Research (B) (Grant/Award Number: 26293197), University of Texas Southwestern Medical Center SPORE (Grant/Award Number: P50CA70907), CPRIT (Grant/Award Number: RP110708). This work was supported, in part, by Grant-in-Aid for Exploratory Research 26670416 and Grant-in-Aid for Scientific Research (B) 26293197 for M. Sato, a Grant-in-Aid for Scientific Research (C) 23591144 for M. Kondo from the Japan Society for the Promotion of Science, University of Texas Southwestern Medical Center SPORE P50CA70907 for J. Minna, and CPRIT RP110708 for J. Minna. Publisher Copyright: {\textcopyright} 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.",
year = "2017",
month = apr,
doi = "10.1111/cas.13185",
language = "English",
volume = "108",
pages = "732--743",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "4",
}