IGF2 preserves osteosarcoma cell survival by creating an autophagic state of dormancy that protects cells against chemotherapeutic stress

Takatsune Shimizu, Eiji Sugihara, Sayaka Yamaguchi-Iwai, Sakura Tamaki, Yuko Koyama, Walied Kamel, Arisa Ueki, Tomoki Ishikawa, Tatsuyuki Chiyoda, Satoru Osuka, Nobuyuki Onishi, Hiroko Ikeda, Junzo Kamei, Koichi Matsuo, Yumi Fukuchi, Toshihiro Nagai, Junya Toguchida, Yoshiaki Toyama, Akihiro Muto, Hideyuki Saya

研究成果: Article査読

40 被引用数 (Scopus)

抄録

Osteosarcoma is a malignant bone tumor in children and adolescents characterized by intrinsic therapeutic resistance. The IGF2 is expressed at elevated levels in osteosarcoma after treatment with chemotherapy, prompting an examination of its functional contributions to resistance. We found that continuous exposure to IGF2 or insulin in the absence of serum created a dormant growth state in osteosarcoma cells that conferred resistance to various chemotherapeutic drugs in vitro. Mechanistic investigations revealed that this dormant state correlated with downregulation of downstream signaling by the IGF1 receptor, heightened cell survival, enhanced autophagy, and the presence of extracellular glutamine. Notably, inhibiting autophagy or depleting glutamine was sufficient to increase chemotherapeutic sensitivity in osteosarcoma xenografts in mice. Clinically, we confirmed that IGF expression levels were elevated in human osteosarcoma specimens from patients who received chemotherapy. Together, our results suggest that activation of IGF or insulin signaling preserves the survival of osteosarcoma cells under chemotherapeutic stress, providing a drug-resistant population that may engender minimal residual disease. Attenuating this survival mechanism may help overcome therapeutic resistance in osteosarcoma.

本文言語English
ページ(範囲)6531-6541
ページ数11
ジャーナルCancer Research
74
22
DOI
出版ステータスPublished - 15-11-2014
外部発表はい

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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