Immune response of post-transplant peripheral lymphocytes against the patient pre-B cell line, NAGL-1

Masanobu Kasai, Yoshiki Akatsuka, Nobuhiko Emi, Hirofumi Taji, Akio Kohno, Akihiro Abe, Mitsune Tanimoto, Yoshihisa Kodera, Hidehiko Saito

研究成果: Article査読

1 被引用数 (Scopus)

抄録

We have established a pre-B acute lymphoblastic leukemia (ALL) cell line, NAGL-1, from the bone marrow of a patient diagnosed with pre-B ALL. The patient has been disease-free for the 4 years since allogeneic bone marrow transplantation from her HLA-genotypically identical sister. NAGL-1 showed a pre-B cell phenotype (CD19+, CD10+, cμ+, sμ-) mostly identical to freshly isolated leukemic cells from the patient. This cell line strongly expressed HLA class I and HLA-DR molecules, as well as the costimulatory molecules CD54, CD40, and CD86. Cytotoxic T-lymphocyte (CTL) lines were generated by stimulating the donor-derived peripheral blood mononuclear cells with either irradiated leukemic cells or NAGL-1. Both CTL lines showed specific lysis against NAGL-1 in 51Cr release assays. Lytic activity was partially inhibited by anti-CD8 and anti-HLA class I monoclonal antibodies. Treatment of NAGL-1 with TNF-α increased its susceptibility to the CTL line. One CD8+ T cell clone derived from the CTL line killed both the patient phytohemagglutinin (PHA) blasts and NAGL-1 but not the donor PHA blasts, suggesting that the clone recognized the patient-specific minor antigen presented on both PHA blasts and NAGL-1. Utilization of leukemic cell lines could be a useful model for the development of CTL lines and clones for immuno-logical study and potential immunotherapy.

本文言語English
ページ(範囲)112-118
ページ数7
ジャーナルInternational Journal of Hematology
69
2
出版ステータスPublished - 1999

All Science Journal Classification (ASJC) codes

  • Hematology

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