TY - JOUR
T1 - Immunoelectron microscopic localization of decorin in aged human corneal and scleral stroma
AU - Kimura, Satoshi
AU - Kobayashi, Miya
AU - Nakamura, Makoto
AU - Hirano, Koji
AU - Awaya, Shinobu
AU - Hoshino, Takeshi
N1 - Funding Information:
Acknowledgments. This work was in part supported by a grant from the Ministry of Education. Science and Culture of Japan, No. 03-77-1217. We thank Ms. Aki Ohtake for secretarial assistance in preparing the manuscript.
PY - 1995/12
Y1 - 1995/12
N2 - Decorin is one of the major proteoglycans in corneal and scleral stroma. Since the distribution of decorin in the normal human eye has not been fully investigated, we examined its immunoelectron microscopic localization in aged human corneal and scleral stroma using anti-decorin antiserum and gold particle-conjugated secondary antibody. In the corneal stroma, decorin was located on the surface of collagen fibrils, on interfibrillar type VI collagen filaments and on dark cross-bands of long-spacing collagen which occurred in the aged human corneal stroma. In the scleral stroma where the banding pattern of D-periodic collagen fibrils was clearly observed, decorin was located on the surface of D-periodic collagen fibrils, chiefly on the d (XI) and e (IX, X) bands, and also on interfibrillar amorphous materials of moderate density which were labeled with anti-type VI collagen antiserum.
AB - Decorin is one of the major proteoglycans in corneal and scleral stroma. Since the distribution of decorin in the normal human eye has not been fully investigated, we examined its immunoelectron microscopic localization in aged human corneal and scleral stroma using anti-decorin antiserum and gold particle-conjugated secondary antibody. In the corneal stroma, decorin was located on the surface of collagen fibrils, on interfibrillar type VI collagen filaments and on dark cross-bands of long-spacing collagen which occurred in the aged human corneal stroma. In the scleral stroma where the banding pattern of D-periodic collagen fibrils was clearly observed, decorin was located on the surface of D-periodic collagen fibrils, chiefly on the d (XI) and e (IX, X) bands, and also on interfibrillar amorphous materials of moderate density which were labeled with anti-type VI collagen antiserum.
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M3 - Article
C2 - 8991920
AN - SCOPUS:0029421352
SN - 2050-5698
VL - 44
SP - 445
EP - 449
JO - Microscopy (Oxford, England)
JF - Microscopy (Oxford, England)
IS - 6
ER -