Immunohistochemical analysis of arterial wall cellular infiltration in Buerger's disease (endarteritis obliterans)

M. Kobayashi, M. Ito, A. Nakagawa, N. Nishikimi, Y. Nimura

研究成果: Article査読

83 被引用数 (Scopus)

抄録

Purpose: The diagnosis of Buerger's disease has depended on clinical symptoms and angiographic findings, whereas pathologic findings are considered to be of secondary importance. Arteries from patients with Buerger's tissue were analyzed histologically, including immunophenotyping of the infiltrating cells, to elucidate the nature of Buerger's disease as a vasculitis. Methods: Thirty-three specimens from nine patients, in whom Buerger's disease was diagnosed on the basis of our clinical and angiographic criteria between 1980 and 1995 at Nagoya University Hospital, were studied. Immunohistochemical Studies were performed on paraffin-embedded tissue with a labeled streptoavidin-biotin method. Results: The general architecture of vessel walls was well preserved regardless of the stage of disease, and cell infiltration was observed mainly in the thrombus and the intima. Among infiltrating cells, CD3+ T cells greatly outnumbered CD20+ B cells. CD68+ macrophages or S-100+ dendritic cells were detected, especially in the intima during acute and subacute stages. All cases except one showed infiltration by the human leukocyte antigen-D region (HLA-DR) antigen- bearing macrophages and dendritic cells in the intima. Immunoglobulins G, A, and M (IgG, IgA, IgM) and complement factors 3d and 4c (C3d, C4c) were deposited along the internal elastic lamina. Conclusion: Buerger's disease is strictly an endarteritis that is introduced by T-cell mediated cellular immunity and by B-cell mediated humoral immunity associated with activation of macrophages or dendritic cells in the intima.

本文言語English
ページ(範囲)451-458
ページ数8
ジャーナルJournal of Vascular Surgery
29
3
DOI
出版ステータスPublished - 1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • 外科
  • 循環器および心血管医学

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