Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers

Yoshiharu Miyamoto, Ryo Hosotani, Michihiko Wada, Jeon Uk Lee, Takatomo Koshiba, Koji Fujimoto, Shouichirou Tsuji, Sanae Nakajima, Ryuichiro Doi, Masayuki Kato, Yuta Shimada, Masayuki Imamura

研究成果: Article

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Expression of several members of the Bcl-2 family proteins was investigated by means of both immunohistochemical analysis in 30 invasive ductal adenocarcinomas and 23 intraductal papillary-mucinous tumors (IPMTs) and immunoblot analysis in 6 cancer tissues and 7 pancreatic cancer cell lines. We found that Bcl-2 was expressed in 23%, Bax in 53%, Bcl-X in 90%, and Mcl-1 in 90% of the invasive ductal adenocarcinomas. In intraductal papillary-mucinous adenocarcinomas, the expression rate of Bax was 44% and those of Bcl-XL and Mcl-1 were 88%; these values were higher than those for intraductal papillary-mucinous adenomas. Immunoblot analysis indentified Bcl-XL as the predominant form of the Bcl-X protein in both pancreatic cancer tissues and cell lines, and demonstrated that both Bcl-XL and Mcl-1 protein levels were uniformly high in all cell lines. These results suggest that an imbalance between antiapoptosis proteins (such as Bcl-2, Bcl-XL, and Mcl-1) and proapoptotic proteins (such as Bax and Bcl-Xs) is involved in the distinctive biologic features of adenocarcinomas of the pancreas. Furthermore, predominantly high expressions of Bcl-XL and Mcl-1 in intraductal papillary-mucinous adenocarcinomas might be involved in the carcinogenesis in IPMT of the pancreas.

元の言語English
ページ(範囲)73-82
ページ数10
ジャーナルOncology
56
発行部数1
DOI
出版物ステータスPublished - 01-01-1999

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

フィンガープリント Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Miyamoto, Y., Hosotani, R., Wada, M., Lee, J. U., Koshiba, T., Fujimoto, K., Tsuji, S., Nakajima, S., Doi, R., Kato, M., Shimada, Y., & Imamura, M. (1999). Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers. Oncology, 56(1), 73-82. https://doi.org/10.1159/000011933