Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers

Yoshiharu Miyamoto, Ryo Hosotani, Michihiko Wada, Jeon Uk Lee, Takatomo Koshiba, Koji Fujimoto, Shouichirou Tsuji, Sanae Nakajima, Ryuichiro Doi, Masayuki Kato, Yuta Shimada, Masayuki Imamura

研究成果: Article

137 引用 (Scopus)

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Expression of several members of the Bcl-2 family proteins was investigated by means of both immunohistochemical analysis in 30 invasive ductal adenocarcinomas and 23 intraductal papillary-mucinous tumors (IPMTs) and immunoblot analysis in 6 cancer tissues and 7 pancreatic cancer cell lines. We found that Bcl-2 was expressed in 23%, Bax in 53%, Bcl-X in 90%, and Mcl-1 in 90% of the invasive ductal adenocarcinomas. In intraductal papillary-mucinous adenocarcinomas, the expression rate of Bax was 44% and those of Bcl-XL and Mcl-1 were 88%; these values were higher than those for intraductal papillary-mucinous adenomas. Immunoblot analysis indentified Bcl-XL as the predominant form of the Bcl-X protein in both pancreatic cancer tissues and cell lines, and demonstrated that both Bcl-XL and Mcl-1 protein levels were uniformly high in all cell lines. These results suggest that an imbalance between antiapoptosis proteins (such as Bcl-2, Bcl-XL, and Mcl-1) and proapoptotic proteins (such as Bax and Bcl-Xs) is involved in the distinctive biologic features of adenocarcinomas of the pancreas. Furthermore, predominantly high expressions of Bcl-XL and Mcl-1 in intraductal papillary-mucinous adenocarcinomas might be involved in the carcinogenesis in IPMT of the pancreas.

元の言語English
ページ(範囲)73-82
ページ数10
ジャーナルOncology
56
発行部数1
DOI
出版物ステータスPublished - 01-01-1999

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Pancreatic Neoplasms
Papillary Adenocarcinoma
Mucinous Adenocarcinoma
Adenocarcinoma
Cell Line
Pancreas
Proteins
bcl-X Protein
Neoplasms
Adenoma
Carcinogenesis

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Miyamoto, Y., Hosotani, R., Wada, M., Lee, J. U., Koshiba, T., Fujimoto, K., ... Imamura, M. (1999). Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers. Oncology, 56(1), 73-82. https://doi.org/10.1159/000011933
Miyamoto, Yoshiharu ; Hosotani, Ryo ; Wada, Michihiko ; Lee, Jeon Uk ; Koshiba, Takatomo ; Fujimoto, Koji ; Tsuji, Shouichirou ; Nakajima, Sanae ; Doi, Ryuichiro ; Kato, Masayuki ; Shimada, Yuta ; Imamura, Masayuki. / Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers. :: Oncology. 1999 ; 巻 56, 番号 1. pp. 73-82.
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title = "Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers",
abstract = "Expression of several members of the Bcl-2 family proteins was investigated by means of both immunohistochemical analysis in 30 invasive ductal adenocarcinomas and 23 intraductal papillary-mucinous tumors (IPMTs) and immunoblot analysis in 6 cancer tissues and 7 pancreatic cancer cell lines. We found that Bcl-2 was expressed in 23{\%}, Bax in 53{\%}, Bcl-X in 90{\%}, and Mcl-1 in 90{\%} of the invasive ductal adenocarcinomas. In intraductal papillary-mucinous adenocarcinomas, the expression rate of Bax was 44{\%} and those of Bcl-XL and Mcl-1 were 88{\%}; these values were higher than those for intraductal papillary-mucinous adenomas. Immunoblot analysis indentified Bcl-XL as the predominant form of the Bcl-X protein in both pancreatic cancer tissues and cell lines, and demonstrated that both Bcl-XL and Mcl-1 protein levels were uniformly high in all cell lines. These results suggest that an imbalance between antiapoptosis proteins (such as Bcl-2, Bcl-XL, and Mcl-1) and proapoptotic proteins (such as Bax and Bcl-Xs) is involved in the distinctive biologic features of adenocarcinomas of the pancreas. Furthermore, predominantly high expressions of Bcl-XL and Mcl-1 in intraductal papillary-mucinous adenocarcinomas might be involved in the carcinogenesis in IPMT of the pancreas.",
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Miyamoto, Y, Hosotani, R, Wada, M, Lee, JU, Koshiba, T, Fujimoto, K, Tsuji, S, Nakajima, S, Doi, R, Kato, M, Shimada, Y & Imamura, M 1999, 'Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers', Oncology, 巻. 56, 番号 1, pp. 73-82. https://doi.org/10.1159/000011933

Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers. / Miyamoto, Yoshiharu; Hosotani, Ryo; Wada, Michihiko; Lee, Jeon Uk; Koshiba, Takatomo; Fujimoto, Koji; Tsuji, Shouichirou; Nakajima, Sanae; Doi, Ryuichiro; Kato, Masayuki; Shimada, Yuta; Imamura, Masayuki.

:: Oncology, 巻 56, 番号 1, 01.01.1999, p. 73-82.

研究成果: Article

TY - JOUR

T1 - Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers

AU - Miyamoto, Yoshiharu

AU - Hosotani, Ryo

AU - Wada, Michihiko

AU - Lee, Jeon Uk

AU - Koshiba, Takatomo

AU - Fujimoto, Koji

AU - Tsuji, Shouichirou

AU - Nakajima, Sanae

AU - Doi, Ryuichiro

AU - Kato, Masayuki

AU - Shimada, Yuta

AU - Imamura, Masayuki

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Expression of several members of the Bcl-2 family proteins was investigated by means of both immunohistochemical analysis in 30 invasive ductal adenocarcinomas and 23 intraductal papillary-mucinous tumors (IPMTs) and immunoblot analysis in 6 cancer tissues and 7 pancreatic cancer cell lines. We found that Bcl-2 was expressed in 23%, Bax in 53%, Bcl-X in 90%, and Mcl-1 in 90% of the invasive ductal adenocarcinomas. In intraductal papillary-mucinous adenocarcinomas, the expression rate of Bax was 44% and those of Bcl-XL and Mcl-1 were 88%; these values were higher than those for intraductal papillary-mucinous adenomas. Immunoblot analysis indentified Bcl-XL as the predominant form of the Bcl-X protein in both pancreatic cancer tissues and cell lines, and demonstrated that both Bcl-XL and Mcl-1 protein levels were uniformly high in all cell lines. These results suggest that an imbalance between antiapoptosis proteins (such as Bcl-2, Bcl-XL, and Mcl-1) and proapoptotic proteins (such as Bax and Bcl-Xs) is involved in the distinctive biologic features of adenocarcinomas of the pancreas. Furthermore, predominantly high expressions of Bcl-XL and Mcl-1 in intraductal papillary-mucinous adenocarcinomas might be involved in the carcinogenesis in IPMT of the pancreas.

AB - Expression of several members of the Bcl-2 family proteins was investigated by means of both immunohistochemical analysis in 30 invasive ductal adenocarcinomas and 23 intraductal papillary-mucinous tumors (IPMTs) and immunoblot analysis in 6 cancer tissues and 7 pancreatic cancer cell lines. We found that Bcl-2 was expressed in 23%, Bax in 53%, Bcl-X in 90%, and Mcl-1 in 90% of the invasive ductal adenocarcinomas. In intraductal papillary-mucinous adenocarcinomas, the expression rate of Bax was 44% and those of Bcl-XL and Mcl-1 were 88%; these values were higher than those for intraductal papillary-mucinous adenomas. Immunoblot analysis indentified Bcl-XL as the predominant form of the Bcl-X protein in both pancreatic cancer tissues and cell lines, and demonstrated that both Bcl-XL and Mcl-1 protein levels were uniformly high in all cell lines. These results suggest that an imbalance between antiapoptosis proteins (such as Bcl-2, Bcl-XL, and Mcl-1) and proapoptotic proteins (such as Bax and Bcl-Xs) is involved in the distinctive biologic features of adenocarcinomas of the pancreas. Furthermore, predominantly high expressions of Bcl-XL and Mcl-1 in intraductal papillary-mucinous adenocarcinomas might be involved in the carcinogenesis in IPMT of the pancreas.

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Miyamoto Y, Hosotani R, Wada M, Lee JU, Koshiba T, Fujimoto K その他. Immunohistochemical analysis of Bcl-2, Bax, Bcl-X, and Mcl-1 expression in pancreatic cancers. Oncology. 1999 1 1;56(1):73-82. https://doi.org/10.1159/000011933