Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells

Shota Fujii, Satoru Yoshida, Emi Inagaki, Shin Hatou, Kazuo Tsubota, Masayo Takahashi, Shigeto Shimmura, Sunao Sugita

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Collecting sufficient quantities of primary neural crest cells (NCCs) for experiments is difficult, as NCCs are embryonic transient tissue that basically does not proliferate. We successfully induced NCCs from human induced pluripotent stem cells (iPSCs) in accordance with a previously described method with some modifications. The protocol used in this study efficiently produced large amounts of iPSC-derived NCCs (iPSC-NCCs). Many researchers have recently produced large amounts of iPSC-NCCs and used these to examine the physiological properties, such as migratory activity, and the potential for medical uses such as wound healing. Immunological properties of NCCs are yet to be reported. Therefore, the purpose of this study was to assess the immunological properties of human iPSC-NCCs. Our current study showed that iPSC-NCCs were hypoimmunogenic and had immunosuppressive properties in vitro. Expression of HLA class I molecules on iPSC-NCCs was lower than that observed for iPSCs, and there was no expression of HLA class II and costimulatory molecules on the cells. With regard to the immunosuppressive properties, iPSC-NCCs greatly inhibited T cell activation (cell proliferation and production of inflammatory cytokines) after stimulation. iPSC-NCCs constitutively expressed membrane-bound TGF-β, and TGF-β produced by iPSC-NCCs played a critical role in T cell suppression. Thus, cultured human NCCs can fully suppress T cell activation in vitro. This study may contribute to the realization of using stem cell-derived NCCs in cell-based medicine.

本文言語English
ページ(範囲)28-43
ページ数16
ジャーナルStem Cells and Development
28
1
DOI
出版ステータスPublished - 01-01-2019

All Science Journal Classification (ASJC) codes

  • 血液学
  • 発生生物学
  • 細胞生物学

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