Impaired DNA replication prompts deletions within palindromic sequences, but does not induce translocations in human cells

Hiroki Kurahashi, Hidehito Inagaki, Takema Kato, Eriko Hosoba, Hiroshi Kogo, Tamae Ohye, Makiko Tsutsumi, Hasbaira Bolor, Maoqing Tong, Beverly S. Emanuel

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Palindromic regions are unstable and susceptible to deletion in prokaryotes and eukaryotes possibly due to stalled or slow replication. In the human genome, they also appear to become partially or completely deleted, while two palindromic AT-rich repeats (PATRR) contribute to known recurrent constitutional translocations. To explore the mechanism that causes the development of palindrome instabilities in humans, we compared the incidence of de novo translocations and deletions at PATRRs in human cells. Using a highly sensitive PCR assay that can detect single molecules, de novo deletions were detected neither in human somatic cells nor in sperm. However, deletions were detected at low frequency in cultured cell lines. Inhibition of DNA replication by administration of siRNA against the DNA polymerase alpha 1 (POLA1) gene or introduction of POLA inhibitors increased the frequency. This is in contrast to PATRR-mediated translocations that were never detected in similar conditions but were observed frequently in human sperm samples. Further deletions were found to take place during both leading- and lagging-strand synthesis. Our data suggest that stalled or slow replication induces deletions within PATRRs, but that other mechanisms might contribute to PATRR-mediated recurrent translocations in humans.

本文言語English
ページ(範囲)3397-3406
ページ数10
ジャーナルHuman molecular genetics
18
18
DOI
出版ステータスPublished - 2009

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学
  • 遺伝学(臨床)

フィンガープリント

「Impaired DNA replication prompts deletions within palindromic sequences, but does not induce translocations in human cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル