In vitro analysis of potency restriction during epiblast differentiation

Mito Kanatsu, Nobuyuki Takakura, Hiroshi Kataoka, Kunihiro Tsuchida, Satomi Nishikawa, Shin Ichi Nishikawa

研究成果: Article

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During mammalian gastrulation, posterior part of primitive ectoderm differentiates into subsets of mesoderm cells, the most proximal portion of which subsequently gives rise to vascular endothelial cells and hematopoietic cells. To analyze this process in which the potency of multipotent epiblast cells is restricted progressively into hematopoietic cell lineages, we have established a culture system that allows differentiation of epiblast cells as well as embryonic stem cells into hematopoietic cells. Using this culture system, we showed that all parts of epiblast tissues at early streak and late bud stages preserved hematogenic potency, while it was lost from anterior region at early head fold stages. However, this loss of hematogenic potency in the anterior region of the head fold stage embryo was reinduced by addition of activin in the culture. Moreover, in order to detect transitory stages of mesoderm cells that are the direct progeny of multipotent epiblast cells, we developed three monoclonal antibodies that are able to define distinct subsets of mesoderm cells in the gastrulating embryo. These results are discussed from a view of cell-mass based commitment.

元の言語English
ページ(範囲)457-459
ページ数3
ジャーナルLeukemia
11
発行部数SUPPL. 3
出版物ステータスPublished - 1997

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

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    Kanatsu, M., Takakura, N., Kataoka, H., Tsuchida, K., Nishikawa, S., & Nishikawa, S. I. (1997). In vitro analysis of potency restriction during epiblast differentiation. Leukemia, 11(SUPPL. 3), 457-459.