In vitro Evaluation of Linezolid and Doripenem Clearance with Different Hemofilters

Toshihisa Hiraiwa, Kazuhiro Moriyama, Kana Matsumoto, Yasuyo Shimomura, Yu Kato, Chizuru Yamashita, Yoshitaka Hara, Takahiro Kawaji, Yasuyoshi Kurimoto, Tomoyuki Nakamura, Naohide Kuriyama, Junpei Shibata, Hidefumi Komura, Kunihiko Morita, Osamu Nishida

研究成果: Article

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Introduction: Renal replacement therapy (RRT) is widely used in the treatment of septic acute kidney injury. However, little is known about how the adsorption properties of hemofilters used in RRT affect antibiotic concentration. Because a cytokine-adsorption membrane is frequently used in RRT, it is important to determine the antibiotic adsorption capacity of this membrane. Objective: The present study aimed to investigate the antibiotic adsorption capacity of different hemofilter membranes by in vitro experiments using 2 antibacterial agents (linezolid and doripenem). Methods: We performed experimental hemofiltration in vitro using polyacrylonitrile (AN69ST), polymethylmethacrylate (PMMA), and polysulfone (PS) hemofilters for 1,440 min. The test solution was a 1,000-mL substitution fluid containing 30 μg/mL linezolid and 120 μg/mL doripenem. We measured drug concentrations at the inlet, outlet, and filtrate ports of the hemofilters for 1,440 min and calculated the sieving coefficient (SC) and adsorption rate (Ra) of the drugs onto the hemofilters. Results: The amount of linezolid adsorbed onto AN69ST, PMMA, and PS membranes was decreased relative to that in the control group at 15 min (p < 0.05). However, no SC for linezolid was obtained thereafter. The Ra of linezolid onto AN69ST, PMMA, and PS membranes was higher than that in the control group (p < 0.05). In contrast, no significant differences were observed in the concentrations and Ra values of doripenem adsorbed onto AN69ST, PMMA, and PS membranes compared with those in the control group. Conclusions: Doripenem was not adsorbed onto PMMA, PS, and AN69ST membranes. Linezolid was adsorbed onto PMMA, PS, and AN69ST membranes, but only temporarily, and this did not affect drug bioavailability.

元の言語English
ジャーナルBlood Purification
DOI
出版物ステータスAccepted/In press - 01-01-2020

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Hematology
  • Nephrology

これを引用

Hiraiwa, T., Moriyama, K., Matsumoto, K., Shimomura, Y., Kato, Y., Yamashita, C., Hara, Y., Kawaji, T., Kurimoto, Y., Nakamura, T., Kuriyama, N., Shibata, J., Komura, H., Morita, K., & Nishida, O. (受理済み/印刷中). In vitro Evaluation of Linezolid and Doripenem Clearance with Different Hemofilters. Blood Purification. https://doi.org/10.1159/000504039