In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA

Christi L. McElheny; Erin L. Fowler; Alina Iovleva; Ryan K. Shields; Yohei Doi

doi:10.1128/Spectrum.01779-21

In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA

Christi L. McElheny
, Erin L. Fowler
, Alina Iovleva
, Ryan K. Shields
, Yohei Doi

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

70 被引用数 (Scopus)

抄録

By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.

本文言語	英語
論文番号	e01779-21
ジャーナル	Microbiology spectrum
巻	9
号	3
DOI	https://doi.org/10.1128/Spectrum.01779-21
出版ステータス	出版済み - 12-2021
外部発表	はい

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この成果は、次の持続可能な開発目標に貢献しています

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All Science Journal Classification (ASJC) codes

生理学
生態学
免疫学および微生物学一般
遺伝学
微生物学（医療）
細胞生物学
感染症

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10.1128/Spectrum.01779-21

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title = "In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA",

abstract = "By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.",

author = "McElheny, \{Christi L.\} and Fowler, \{Erin L.\} and Alina Iovleva and Shields, \{Ryan K.\} and Yohei Doi",

note = "Publisher Copyright: {\textcopyright} 2021 McElheny et al.",

year = "2021",

month = dec,

doi = "10.1128/Spectrum.01779-21",

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T1 - In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA

AU - McElheny, Christi L.

AU - Fowler, Erin L.

AU - Iovleva, Alina

AU - Shields, Ryan K.

AU - Doi, Yohei

PY - 2021/12

Y1 - 2021/12

N2 - By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.

AB - By serially exposing an NDM-producing Klebsiella pneumoniae clinical strain to cefiderocol, we obtained a mutant with cefiderocol MIC of .128 mg/ml. The mutant contained an early stop codon in the iron transporter gene cirA, and its complementation fully restored susceptibility. The cirA-deficient mutant was competed out by the parental strain in vitro, suggesting reduced fitness.

UR - https://www.scopus.com/pages/publications/85122737596

UR - https://www.scopus.com/pages/publications/85122737596#tab=citedBy

U2 - 10.1128/Spectrum.01779-21

DO - 10.1128/Spectrum.01779-21

M3 - Article

C2 - 34756080

AN - SCOPUS:85122737596

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JO - Microbiology spectrum

JF - Microbiology spectrum

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ER -

In Vitro Evolution of Cefiderocol Resistance in an NDMProducing Klebsiella pneumoniae Due to Functional Loss of CirA

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UN SDG

All Science Journal Classification (ASJC) codes

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