In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos

Shin Ichi Nishikawa, Satomi Nishikawa, Hiroshi Kawamoto, Hisahiro Yoshida, Masami Kizumoto, Hiroshi Kataoka, Yoshimoto Katsura

研究成果: Article査読

296 被引用数 (Scopus)

抄録

We have investigated the lymphohematopoietic potentials of endothelial cells (EC) and hematopoietic cells (HPC) sorted from embryos. Expression of VE-cadherin, CD45, and Ter119 was used to distinguish EC (VE- cadherin+CD45-Ter119-) from HPC (VE-cadherin-CD45+). Thus defined, EC population takes up acetylated LDL and coexpresses CD31, Flk1, and CD34. In E9.5 embryos, EC from yolk sac (YS) and the embryo proper generate blood cells, including lymphocytes. Thus, lymphohematopoietic EC do exist in the embryo, and they are generated both in YS and the embryo proper. On the other hand, HPC with lymphopoietic potency appear first in the embryo proper. These findings implicate involvement of multiple environmental cues for acquiring lymphopoietic competency during differentiation of HPC.

本文言語English
ページ(範囲)761-769
ページ数9
ジャーナルImmunity
8
6
DOI
出版ステータスPublished - 06-1998
外部発表はい

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学
  • 感染症

フィンガープリント

「In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル