In vitro positron emission tomography (PET): use of positron emission tracers in functional imaging in living brain slices

Kiyoshi Matsumura, Mats Bergström, Hirotaka Onoe, Hajime Takechi, Göran Westerberg, Gunnar Antoni, Peter Bjurling, Gunilla B. Jacobson, Bengt Långström, Yasuyoshi Watanabe

研究成果: Article査読

38 被引用数 (Scopus)

抄録

Positron-emitting radionuclides have short half-lives and high radiation energies compared with radioisotopes generally used in biomedical research. We examined the possibility of applying positron emitter-labeled compounds to functional imaging in brain slices kept viable in an oxygenated buffer solution. Brain slices (300 μm thick) containing the striatum were incubated with positron emitter-labeled tracers for 30-45 min. The slices were then rinsed and placed on the bottom of a Plexiglas chamber filled with oxygenated Krebs-Ringer solution. The bottom of the chamber consisted of a thin polypropylene film to allow good penetration of β+ particles from the brain slices. The chamber was placed on a storage phosphor screen, which has a higher sensitivity and a wider dynamic range than X-ray films. After an exposure period of 15-60 min, the screen was scanned by the analyzer and radioactivity images of brain slices were obtained within 20 min. We succeeded in obtaining quantitative images of (1) [18F]fluoro-deoxyglucose uptake, (2) dopamine D2 receptor binding, (3) dopa-decarboxylase activity, and (4) release of [ 11 C]dopamine preloaded as l-[11C]DOPA in the brain slice preparation. These results demonstrate that positron emitter-labeled tracers in combination with storage phosphor screens are useful for functional imaging of living brain slices as a novel neuroscience technique.

本文言語English
ページ(範囲)219-229
ページ数11
ジャーナルNeuroscience Research
22
2
DOI
出版ステータスPublished - 05-1995
外部発表はい

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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