In Vitro Splicing Assays

Akila Mayeda, Adrian R. Krainer

研究成果: Chapter

4 引用 (Scopus)

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In vitro splicing assays represent an important technique to analyze, in detail, the basic splicing mechanisms for a pre-mRNA of interest. The cell-free assays, under defined conditions, have many advantages for characterizing the cis-acting elements and trans-acting factors responsible for the regulation of alternative splicing. Radiolabeled transcripts of minigenes of interest are spliced in HeLa cell nuclear extracts under various conditions, and the splicing products analyzed directly using denaturing polyacrylamide gel electrophoresis, or detected indirectly using RT-PCR. Here, a standard method of in vitro splicing is described that has been used successfully for many years in the authors' laboratories.

元の言語English
ホスト出版物のタイトルAlternative pre-mRNA Splicing
ホスト出版物のサブタイトルTheory and Protocols
出版者Wiley-VCH
ページ320-329
ページ数10
ISBN(印刷物)9783527326068
DOI
出版物ステータスPublished - 02-02-2012

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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    Mayeda, A., & Krainer, A. R. (2012). In Vitro Splicing Assays. : Alternative pre-mRNA Splicing: Theory and Protocols (pp. 320-329). Wiley-VCH. https://doi.org/10.1002/9783527636778.ch30