In Vitro Splicing Assays

Akila Mayeda, Adrian R. Krainer

研究成果: Chapter

4 引用 (Scopus)


In vitro splicing assays represent an important technique to analyze, in detail, the basic splicing mechanisms for a pre-mRNA of interest. The cell-free assays, under defined conditions, have many advantages for characterizing the cis-acting elements and trans-acting factors responsible for the regulation of alternative splicing. Radiolabeled transcripts of minigenes of interest are spliced in HeLa cell nuclear extracts under various conditions, and the splicing products analyzed directly using denaturing polyacrylamide gel electrophoresis, or detected indirectly using RT-PCR. Here, a standard method of in vitro splicing is described that has been used successfully for many years in the authors' laboratories.

ホスト出版物のタイトルAlternative pre-mRNA Splicing
ホスト出版物のサブタイトルTheory and Protocols
出版物ステータスPublished - 02-02-2012

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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  • これを引用

    Mayeda, A., & Krainer, A. R. (2012). In Vitro Splicing Assays. : Alternative pre-mRNA Splicing: Theory and Protocols (pp. 320-329). Wiley-VCH.