In vitro study of endotoxin adsorption by a polymyxin b-immobilized fiber column

Chizuru Yamashita, Kazuhiro Moriyama, Daisuke Hasegawa, Yu Kato, Toshikazu Sakai, Takahiro Kawaji, Yasuyo Shimomura, Yasuyoshi Kurimoto, Mariko Nagata, Osamu Nishida

研究成果: Article

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Background: Polymyxin B-immobilized fiber (PMX-F) columns are used as therapeutic interventions for septic shock. The clinical efficacy has been reported for 2-h applications, but their ability to adsorb endotoxin over longer treatments has not been fully elucidated. We hypothesized that PMX-F columns are capable of endotoxin removal for more than 2 h. Method: We designed closed circuits incorporating either a PMX-F column with an 8.5-mL priming volume (PMX-01R) or a sham-control column, and used inactivated fetal bovine serum as the circulating perfusate. Endotoxin was continuously injected at a fixed rate for 24 h, and perfusate endotoxin concentrations were measured at fixed time points. PMX-01R endotoxin adsorption was calculated from the difference in the endotoxin concentrations. Results: PMX-01R endotoxin adsorption increased continuously in a virtually linear manner. Conclusions: The PMX-01R column showed sustained endotoxin adsorption for at least 24 h. This indicated that PMX-F columns would be capable of clinical endotoxin removal for 24 h.

元の言語English
ページ(範囲)269-273
ページ数5
ジャーナルBlood Purification
46
発行部数4
DOI
出版物ステータスPublished - 01-10-2018

All Science Journal Classification (ASJC) codes

  • Hematology
  • Nephrology

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    Yamashita, C., Moriyama, K., Hasegawa, D., Kato, Y., Sakai, T., Kawaji, T., Shimomura, Y., Kurimoto, Y., Nagata, M., & Nishida, O. (2018). In vitro study of endotoxin adsorption by a polymyxin b-immobilized fiber column. Blood Purification, 46(4), 269-273. https://doi.org/10.1159/000489920